6E1O

afTMEM16 reconstituted in nanodiscs in the presence of Ca2+ and ceramide 24:0

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth B]	SCOP2B Superfamily	Anoctamin-like	8099514	3002639	SCOP2B (2022-06-29)
B [auth A]	SCOP2 Family	Anoctamin-like	8099513	4004881	SCOP2 (2022-06-29)
B [auth A]	SCOP2 Superfamily	Anoctamin-like	8099514	3002639	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth B]	PF04547	e6e1oB1	A: alpha bundles	X: TMEM16 lipid scramblase transmembrane domain (From Topology)	H: TMEM16 lipid scramblase transmembrane domain (From Topology)	T: TMEM16 lipid scramblase transmembrane domain	F: PF04547	ECOD (1.6)
A [auth B]	PF20877	e6e1oB2	A: a+b two layers	X: Alpha-beta plaits	H: Alpha-beta plait domain in TMEM16 lipid scramblase (From Topology)	T: Alpha-beta plait domain in TMEM16 lipid scramblase	F: PF20877	ECOD (1.6)
B [auth A]	PF04547	e6e1oA1	A: alpha bundles	X: TMEM16 lipid scramblase transmembrane domain (From Topology)	H: TMEM16 lipid scramblase transmembrane domain (From Topology)	T: TMEM16 lipid scramblase transmembrane domain	F: PF04547	ECOD (1.6)
B [auth A]	PF20877	e6e1oA2	A: a+b two layers	X: Alpha-beta plaits	H: Alpha-beta plait domain in TMEM16 lipid scramblase (From Topology)	T: Alpha-beta plait domain in TMEM16 lipid scramblase	F: PF20877	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B], B [auth A]	PF20877	Alpha-beta plait domain in TMEM16 lipid scramblase (Anoctamin_N)	Alpha-beta plait domain in TMEM16 lipid scramblase	This entry represents an alpha-beta plait domain from a group of fungal TMEM16 family of proteins, also known as anoctamins, including SwissProt:Q4WA18 from Aspergillus fumigatus and ist2 from S.pombe. Proteins in this entry operate as Ca2+-activated ...	This entry represents an alpha-beta plait domain from a group of fungal TMEM16 family of proteins, also known as anoctamins, including SwissProt:Q4WA18 from Aspergillus fumigatus and ist2 from S.pombe. Proteins in this entry operate as Ca2+-activated lipid scramblases [1-3]. This domain may play a role in dimerisation, although its precise function is uncertain.	Domain
A [auth B], B [auth A]	PF04547	Calcium-activated chloride channel (Anoctamin)	Calcium-activated chloride channel	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B], B [auth A]	Plasma membrane channel protein (Aqy1), putative	monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity monoatomic ion channel activity passive transmembrane transporter activity inorganic molecular entity transmembrane transporter activity channel activity monoatomic anion channel activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity inorganic anion transmembrane transporter activity chloride channel activity lipid transporter activity intramembrane lipid transporter activity monoatomic anion transmembrane transporter activity chloride transmembrane transporter activity monoatomic ion channel activity passive transmembrane transporter activity inorganic molecular entity transmembrane transporter activity channel activity monoatomic anion channel activity monoatomic ion transmembrane transporter activity transporter activity transmembrane transporter activity inorganic anion transmembrane transporter activity chloride channel activity lipid transporter activity intramembrane lipid transporter activity phospholipid scramblase activity phospholipid transporter activity calcium channel activity monoatomic cation transmembrane transporter activity voltage-gated calcium channel activity gated channel activity voltage-gated channel activity voltage-gated monoatomic ion channel activity voltage-gated monoatomic cation channel activity monoatomic cation channel activity calcium ion transmembrane transporter activity inorganic cation transmembrane transporter activity metal ion transmembrane transporter activity	transmembrane transport localization transport monoatomic ion transmembrane transport monoatomic ion transport cellular process establishment of localization inorganic ion transmembrane transport inorganic anion transmembrane transport chloride transport chloride transmembrane transport monoatomic anion transport monoatomic anion transmembrane transport inorganic anion transport transmembrane transport localization transport monoatomic ion transmembrane transport monoatomic ion transport cellular process establishment of localization inorganic ion transmembrane transport inorganic anion transmembrane transport chloride transport chloride transmembrane transport monoatomic anion transport monoatomic anion transmembrane transport inorganic anion transport membrane organization phospholipid translocation organic substance transport biological regulation lipid localization phospholipid transport lipid transport lipid translocation regulation of membrane lipid distribution cellular component organization macromolecule localization regulation of biological quality cellular component organization or biogenesis organophosphate ester transport	cell periphery plasma membrane membrane cellular anatomical entity membrane-bounded organelle cell cortex intracellular anatomical structure cortical endoplasmic reticulum organelle subcompartment intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle organelle cell periphery plasma membrane membrane cellular anatomical entity membrane-bounded organelle cell cortex intracellular anatomical structure cortical endoplasmic reticulum organelle subcompartment intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle organelle endoplasmic reticulum cytoplasm endoplasmic reticulum tubular network

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B], B [auth A]	IPR007632	Anoctamin	Family
A [auth B], B [auth A]	IPR049452	Anoctamin, transmembrane domain	Domain
A [auth B], B [auth A]	IPR049456	Anoctamin, alpha-beta plait domain, fungal-type	Domain

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A [auth B], B [auth A]	OPM	Transmembrane	Alpha-helical polytopic	Calcium-activated chloride channel	Anoctamin (TMEM16) family

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A [auth B], B [auth A]	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A [auth B], B [auth A]	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A [auth B], B [auth A]	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	TMEM16 Family Proteins	TMEM16 scramblase in nanodiscs in the presence of Ca²⁺

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.