5JER

Structure of Rotavirus NSP1 bound to IRF-3

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
E [auth A]	d5jera_	All beta proteins	SMAD/FHA domain	SMAD/FHA domain	Interferon regulatory factor 3 (IRF3), transactivation domain	Interferon regulatory factor 3 (IRF3), transactivation domain	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
F [auth C]	d5jerc_	All beta proteins	SMAD/FHA domain	SMAD/FHA domain	Interferon regulatory factor 3 (IRF3), transactivation domain	Interferon regulatory factor 3 (IRF3), transactivation domain	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
G [auth E]	d5jere_	All beta proteins	SMAD/FHA domain	SMAD/FHA domain	Interferon regulatory factor 3 (IRF3), transactivation domain	Interferon regulatory factor 3 (IRF3), transactivation domain	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)
H [auth G]	d5jerg_	All beta proteins	SMAD/FHA domain	SMAD/FHA domain	Interferon regulatory factor 3 (IRF3), transactivation domain	Interferon regulatory factor 3 (IRF3), transactivation domain	Human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
E [auth A]	SCOP2B Superfamily	SMAD/FHA domain-like	8003522	3000137	SCOP2B (2022-06-29)
F [auth C]	SCOP2B Superfamily	SMAD/FHA domain-like	8003522	3000137	SCOP2B (2022-06-29)
G [auth E]	SCOP2B Superfamily	SMAD/FHA domain-like	8003522	3000137	SCOP2B (2022-06-29)
H [auth G]	SCOP2B Superfamily	SMAD/FHA domain-like	8003522	3000137	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
E [auth A]	PF10401	e5jerA1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: PF10401	ECOD (1.6)
F [auth C]	PF10401	e5jerC1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: PF10401	ECOD (1.6)
G [auth E]	PF10401	e5jerE1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: PF10401	ECOD (1.6)
H [auth G]	PF10401	e5jerG1	A: beta sandwiches	X: SMAD/FHA domain (From Topology)	H: SMAD/FHA domain (From Topology)	T: SMAD/FHA domain	F: PF10401	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
E [auth A]	2.60.200.10	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)
F [auth C]	2.60.200.10	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)
G [auth E]	2.60.200.10	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)
H [auth G]	2.60.200.10	Mainly Beta	Sandwich	Tumour Suppressor Smad4		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth B], B [auth D], C [auth F], D [auth H]	PF00981	Rotavirus RNA-binding Protein 53 (NS53) (Rota_NS53)	Rotavirus RNA-binding Protein 53 (NS53)	-	Family
E [auth A], F [auth C], G [auth E], H [auth G]	PF10401	Interferon-regulatory factor 3 (IRF-3)	Interferon-regulatory factor 3	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth B], B [auth D], C [auth F], D [auth H]	Rotavirus NSP1 peptide	RNA binding binding organic cyclic compound binding nucleic acid binding cation binding ion binding metal ion binding small molecule binding	symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms response to stimulus symbiont-mediated suppression of host pathogen-associated molecular pattern receptor signaling pathway response to biotic stimulus biological process involved in symbiotic interaction symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response response to host immune response symbiont-mediated suppression of host defenses symbiont-mediated perturbation of host innate immune response response to host response to external biotic stimulus symbiont-mediated perturbation of host signal transduction pathway symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms response to stimulus symbiont-mediated suppression of host pathogen-associated molecular pattern receptor signaling pathway response to biotic stimulus biological process involved in symbiotic interaction symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response response to host immune response symbiont-mediated suppression of host defenses symbiont-mediated perturbation of host innate immune response response to host response to external biotic stimulus symbiont-mediated perturbation of host signal transduction pathway modulation of process of another organism symbiont-mediated perturbation of host defense response response to host defenses response to other organism symbiont-mediated perturbation of host immune response response to defenses of other organism symbiont-mediated perturbation of host defenses response to external stimulus biological process involved in interaction with host symbiont-mediated suppression of host signal transduction pathway modulation by symbiont of host process cellular process symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity symbiont-mediated suppression of cytoplasmic pattern recognition receptor signaling pathway symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity	host cellular component host intracellular part host cytoskeleton host cell part host intracellular region cellular anatomical entity host intracellular organelle host cell cytoplasm
E [auth A], F [auth C], G [auth E], H [auth G]	Interferon regulatory factor 3	sequence-specific DNA binding DNA binding binding organic cyclic compound binding nucleic acid binding protein-containing complex binding chromatin binding promoter-specific chromatin binding DNA-binding transcription factor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription factor activity sequence-specific double-stranded DNA binding double-stranded DNA binding DNA-binding transcription activator activity, RNA polymerase II-specific sequence-specific DNA binding DNA binding binding organic cyclic compound binding nucleic acid binding protein-containing complex binding chromatin binding promoter-specific chromatin binding DNA-binding transcription factor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription factor activity sequence-specific double-stranded DNA binding double-stranded DNA binding DNA-binding transcription activator activity, RNA polymerase II-specific DNA-binding transcription activator activity identical protein binding protein binding protein domain specific binding DNA-binding transcription repressor activity, RNA polymerase II-specific DNA-binding transcription repressor activity RNA polymerase II transcription regulatory region sequence-specific DNA binding cis-regulatory region sequence-specific DNA binding transcription cis-regulatory region binding transcription regulatory region nucleic acid binding RNA polymerase II cis-regulatory region sequence-specific DNA binding protein homodimerization activity protein dimerization activity	cellular response to stimulus cytoplasmic pattern recognition receptor signaling pathway regulation of defense response positive regulation of response to biotic stimulus regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms regulation of cellular process pattern recognition receptor signaling pathway response to stimulus regulation of response to external stimulus intracellular receptor signaling pathway signaling positive regulation of biological process response to biotic stimulus cell communication cellular response to stimulus cytoplasmic pattern recognition receptor signaling pathway regulation of defense response positive regulation of response to biotic stimulus regulation of response to biotic stimulus biological process involved in interspecies interaction between organisms regulation of cellular process pattern recognition receptor signaling pathway response to stimulus regulation of response to external stimulus intracellular receptor signaling pathway signaling positive regulation of biological process response to biotic stimulus cell communication positive regulation of response to stimulus immune response-activating signaling pathway positive regulation of response to external stimulus immune response-regulating signaling pathway positive regulation of immune response regulation of response to stress response to stress innate immune response regulation of immune system process response to external biotic stimulus signal transduction positive regulation of immune system process innate immune response-activating signaling pathway regulation of biological process positive regulation of innate immune response defense response immune response response to other organism regulation of innate immune response biological regulation defense response to symbiont response to external stimulus regulation of response to stimulus immune system process defense response to other organism positive regulation of defense response activation of innate immune response regulation of immune response cellular process activation of immune response cellular response to stress DNA damage response apoptotic process cell death programmed cell death regulation of apoptotic process regulation of programmed cell death positive regulation of cell communication response to organic substance positive regulation of signal transduction regulation of type I interferon-mediated signaling pathway response to cytokine regulation of signal transduction interferon-mediated signaling pathway cellular response to type I interferon cellular response to cytokine stimulus regulation of response to cytokine stimulus response to chemical response to type I interferon positive regulation of type I interferon-mediated signaling pathway positive regulation of response to cytokine stimulus cytokine-mediated signaling pathway cellular response to organic substance positive regulation of cellular process regulation of signaling cell surface receptor signaling pathway regulation of cytokine-mediated signaling pathway cellular response to chemical stimulus type I interferon-mediated signaling pathway regulation of cell communication positive regulation of cytokine-mediated signaling pathway positive regulation of signaling cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression mRNA transcription mRNA metabolic process macromolecule biosynthetic process nucleic acid metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process organic cyclic compound metabolic process DNA-templated transcription nucleic acid biosynthetic process organic substance metabolic process biosynthetic process organic cyclic compound biosynthetic process organic substance biosynthetic process metabolic process macromolecule metabolic process MDA-5 signaling pathway defense response to virus response to virus multicellular organismal process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process positive regulation of metabolic process positive regulation of biosynthetic process cytokine production regulation of cytokine production regulation of multicellular organismal process positive regulation of macromolecule metabolic process regulation of biosynthetic process positive regulation of type I interferon production type I interferon production regulation of cellular metabolic process regulation of macromolecule metabolic process regulation of cellular biosynthetic process regulation of gene expression positive regulation of gene expression positive regulation of multicellular organismal process regulation of macromolecule biosynthetic process positive regulation of cytokine production regulation of metabolic process positive regulation of cellular biosynthetic process regulation of type I interferon production regulation of primary metabolic process regulation of RNA metabolic process transcription by RNA polymerase II regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process regulation of DNA-templated transcription positive regulation of DNA-templated transcription regulation of RNA biosynthetic process positive regulation of RNA biosynthetic process interferon-alpha production regulation of interferon-alpha production positive regulation of interferon-alpha production positive regulation of canonical NF-kappaB signal transduction intracellular signal transduction regulation of intracellular signal transduction intracellular signaling cassette positive regulation of intracellular signal transduction canonical NF-kappaB signal transduction regulation of canonical NF-kappaB signal transduction immune response-regulating cell surface receptor signaling pathway cell surface toll-like receptor signaling pathway immune response-activating cell surface receptor signaling pathway innate immune response activating cell surface receptor signaling pathway cell surface pattern recognition receptor signaling pathway toll-like receptor 4 signaling pathway response to lipid cellular response to lipopolysaccharide lipopolysaccharide-mediated signaling pathway cellular response to biotic stimulus response to bacterium response to oxygen-containing compound cellular response to molecule of bacterial origin response to lipopolysaccharide cellular response to oxygen-containing compound cellular response to lipid response to molecule of bacterial origin cellular response to nitrogen compound cellular response to dsRNA cellular response to exogenous dsRNA cellular response to organic cyclic compound response to dsRNA response to nitrogen compound response to exogenous dsRNA response to organic cyclic compound toll-like receptor signaling pathway TRIF-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway regulation of interferon-beta production interferon-beta production positive regulation of interferon-beta production antiviral innate immune response regulation of inflammatory response inflammatory response programmed necrotic cell death macrophage apoptotic process myeloid cell apoptotic process leukocyte apoptotic process inflammatory cell apoptotic process cellular response to virus	intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle protein-containing complex protein-DNA complex organelle intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle membrane-bounded organelle mitochondrion intracellular anatomical structure cytoplasm cellular anatomical entity cytosol intracellular organelle protein-containing complex protein-DNA complex organelle intracellular non-membrane-bounded organelle chromatin chromosome non-membrane-bounded organelle membrane-bounded organelle mitochondrion intracellular membrane-bounded organelle nucleus membrane-enclosed lumen intracellular organelle lumen nucleoplasm organelle lumen nuclear lumen

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth B], B [auth D], C [auth F], D [auth H]	IPR002148	Rotavirus non-structural protein 1	Family
E [auth A], F [auth C], G [auth E], H [auth G]	IPR019817	Interferon regulatory factor, conserved site	Conserved Site
E [auth A], F [auth C], G [auth E], H [auth G]	IPR019471	Interferon regulatory factor-3	Domain
E [auth A], F [auth C], G [auth E], H [auth G]	IPR036388	Winged helix-like DNA-binding domain superfamily	Homologous Superfamily
E [auth A], F [auth C], G [auth E], H [auth G]	IPR036390	Winged helix DNA-binding domain superfamily	Homologous Superfamily
E [auth A], F [auth C], G [auth E], H [auth G]	IPR017855	SMAD-like domain superfamily	Homologous Superfamily
E [auth A], F [auth C], G [auth E], H [auth G]	IPR001346	Interferon regulatory factor, DNA-binding domain	Domain
E [auth A], F [auth C], G [auth E], H [auth G]	IPR008984	SMAD/FHA domain superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
E [auth A], F [auth C], G [auth E], H [auth G]	Pharos: Q14653	herpes simplex encephalitis, susceptibility to, 7 Hashimoto thyroiditis Hodgkins lymphoma microphthalmia with limb anomalies rheumatoid arthritis systemic sclerosis Herpes encephalitis hypothyroidism myositis skin basal cell carcinoma vitiligo herpes simplex encephalitis influenza pancreatic ductal adenocarcinoma systemic lupus erythematosus

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.