Annotations: 5EYI

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF19215	e5eyiA2	A: a+b complex topology	X: RNase A-like	H: EndoU-like (From Topology)	T: EndoU-like	F: PF19215	ECOD (1.6)
A	PF22049	e5eyiA1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: PF22049	ECOD (1.6)
B	PF19215	e5eyiB1	A: a+b complex topology	X: RNase A-like	H: EndoU-like (From Topology)	T: EndoU-like	F: PF19215	ECOD (1.6)
B	PF22049	e5eyiB2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: PF22049	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF19215	Coronavirus replicase NSP15, uridylate-specific endoribonuclease (CoV_NSP15_C)	Coronavirus replicase NSP15, uridylate-specific endoribonuclease	This entry represents the C-terminal domain of coronavirus non-structural protein 15 (NSP15 or nsp15). NSP15 is encoded by ORF1a/1ab and proteolytically released from the pp1a/1ab polyprotein. This domain exhibits endoribonuclease activity designated ...	This entry represents the C-terminal domain of coronavirus non-structural protein 15 (NSP15 or nsp15). NSP15 is encoded by ORF1a/1ab and proteolytically released from the pp1a/1ab polyprotein. This domain exhibits endoribonuclease activity designated EndoU, highly conserved in all known CoVs and is part of the replicase-transcriptase complex that plays important roles in virus replication and transcription [1, 2, 3]. NSP15 is a Uridylate-specific endoribonuclease that cleaves the 5'-polyuridines from negative-sense viral RNA, termed PUN RNA either upstream or downstream of uridylates, at GUU or GU to produce molecules with 2',3'-cyclic phosphate ends [2, 3]. PUN RNA is a CoV MDA5-dependent pathogen-associated molecular pattern (PAMP) [3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Non-structural protein 11	peptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity hydrolase activity serine-type endopeptidase activity catalytic activity endopeptidase activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding peptidase activity catalytic activity, acting on a protein serine hydrolase activity serine-type peptidase activity hydrolase activity serine-type endopeptidase activity catalytic activity endopeptidase activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid helicase activity RNA helicase activity ATP-dependent activity, acting on RNA ATP-dependent activity endonuclease activity nuclease activity hydrolase activity, acting on ester bonds 5'-3' RNA polymerase activity RNA polymerase activity transferase activity RNA-dependent RNA polymerase activity nucleotidyltransferase activity transferase activity, transferring phosphorus-containing groups cysteine-type peptidase activity cysteine-type endopeptidase activity lyase activity RNA binding nucleic acid binding ubiquitin-like protein peptidase activity cysteine-type deubiquitinase activity deubiquitinase activity RNA nuclease activity enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase regulator activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity kinase regulator activity pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity, acting on acid anhydrides cation binding transition metal ion binding zinc ion binding metal ion binding	cellular biosynthetic process cellular metabolic process RNA metabolic process viral life cycle primary metabolic process nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process nucleic acid biosynthetic process organic substance metabolic process viral genome replication biosynthetic process cellular biosynthetic process cellular metabolic process RNA metabolic process viral life cycle primary metabolic process nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process organic cyclic compound metabolic process nucleic acid biosynthetic process organic substance metabolic process viral genome replication biosynthetic process viral RNA genome replication organic cyclic compound biosynthetic process viral process organic substance biosynthetic process metabolic process cellular process macromolecule metabolic process symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms symbiont-mediated suppression of host JAK-STAT cascade biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated suppression of host signal transduction pathway symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT activity symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity modulation by symbiont of host process symbiont-mediated perturbation of host signal transduction pathway modulation of process of another organism symbiont-mediated perturbation of host defense response symbiont-mediated suppression of host ISG15-protein conjugation response to host defenses response to stimulus response to other organism symbiont-mediated perturbation of host immune response response to defenses of other organism symbiont-mediated perturbation of host defenses response to external stimulus response to biotic stimulus symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response response to host immune response symbiont-mediated suppression of host defenses symbiont-mediated perturbation of host innate immune response response to host response to external biotic stimulus viral gene expression viral protein processing gene expression DNA-templated transcription symbiont-mediated suppression of host type I interferon-mediated signaling pathway symbiont-mediated suppression of host interferon-mediated signaling pathway symbiont-mediated perturbation of host ubiquitin-like protein modification symbiont-mediated suppression of host PKR/eIFalpha signaling symbiont-mediated perturbation of host translation symbiont-mediated suppression of host-directed shutoff of host translation symbiont-mediated perturbation of host gene expression organonitrogen compound metabolic process proteolysis protein metabolic process	membrane cellular anatomical entity host intracellular membrane-bounded organelle host cellular component host intracellular part host cell cytoplasm host cell part host cell endoplasmic reticulum host intracellular region host cell cytoplasm part host intracellular organelle host cell perinuclear region of cytoplasm host cell membrane host cell nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR008741	Arterivirus papain-like cysteine protease alpha (PCPalpha) domain	Domain
A, B	IPR025773	Arterivirus papain-like cysteine protease beta (PCPbeta) domain	Domain
A, B	IPR032785	Replicase polyprotein 1ab, peptidase C33-associated domain	Domain
A, B	IPR044863	Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain	Domain
A, B	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B	IPR037227	Endoribonuclease EndoU-like	Homologous Superfamily
A, B	IPR009003	Peptidase S1, PA clan	Homologous Superfamily
A, B	IPR032855	Arterivirus polyprotein, nsp2, immunogenic region	Domain
A, B	IPR008760	Equine arteritis virus peptidase S32	Family
A, B	IPR038451	Arterivirus nsp7 alpha superfamily	Homologous Superfamily
A, B	IPR027351	(+) RNA virus helicase core domain	Domain
A, B	IPR023338	Arterivirus NSP4 peptidase domain	Domain
A, B	IPR008743	Arterivirus Nsp2, peptidase C33	Domain
A, B	IPR038155	Arterivirus papain-like cysteine protease alpha (PCPalpha) domain superfamily	Homologous Superfamily
A, B	IPR001205	RNA-directed RNA polymerase, C-terminal domain	Domain
A, B	IPR027355	Nonstructural protein 10, zinc-binding domain, arterivirus	Domain
A, B	IPR046440	Arterivirus Nsp11 N-terminal/Coronavirus NSP15 middle domain	Domain
A, B	IPR044320	NSP11, N-terminal, arterivirus	Domain
A, B	IPR038154	Arterivirus papain-like cysteine protease beta (PCPbeta) domain superfamily	Homologous Superfamily
A, B	IPR043502	DNA/RNA polymerase superfamily	Homologous Superfamily
A, B	IPR044348	Nonstructural protein 10, 1B domain, arterivirus	Domain
A, B	IPR023183	Serine protease, chymotrypsin-like serine protease, C-terminal	Homologous Superfamily
A, B	IPR043609	NendoU domain, nidovirus	Domain
A, B	IPR044314	NSP11, NendoU domain, arterivirus	Domain
A, B	IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	Homologous Superfamily
A, B	IPR007094	RNA-directed RNA polymerase, catalytic domain	Domain
A, B	IPR031932	Arterivirus nonstructural protein 7 alpha	Family