4GUA

Alphavirus P23pro-zbd

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Peptidase_C9	e4guaA1	A: a+b complex topology	X: Cysteine proteinases-like	H: Cysteine proteinases (From Topology)	T: Cysteine proteinases	F: Peptidase_C9	ECOD (1.6)
A	EUF08561	e4guaA4	A: a+b complex topology	X: nsP3 zinc-binding domain (From Topology)	H: nsP3 zinc-binding domain (From Topology)	T: nsP3 zinc-binding domain	F: EUF08561	ECOD (1.6)
A	FtsJ_1	e4guaA2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: FtsJ_1	ECOD (1.6)
A	Macro_3	e4guaA3	A: a/b three-layered sandwiches	X: Macro domain-like (From Topology)	H: Macro domain-like (From Topology)	T: Macro domain-like	F: Macro_3	ECOD (1.6)
B	Peptidase_C9	e4guaB5	A: a+b complex topology	X: Cysteine proteinases-like	H: Cysteine proteinases (From Topology)	T: Cysteine proteinases	F: Peptidase_C9	ECOD (1.6)
B	EUF08561	e4guaB6	A: a+b complex topology	X: nsP3 zinc-binding domain (From Topology)	H: nsP3 zinc-binding domain (From Topology)	T: nsP3 zinc-binding domain	F: EUF08561	ECOD (1.6)
B	FtsJ_1	e4guaB1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: FtsJ_1	ECOD (1.6)
B	Macro_3	e4guaB7	A: a/b three-layered sandwiches	X: Macro domain-like (From Topology)	H: Macro domain-like (From Topology)	T: Macro domain-like	F: Macro_3	ECOD (1.6)
C	Peptidase_C9	e4guaC5	A: a+b complex topology	X: Cysteine proteinases-like	H: Cysteine proteinases (From Topology)	T: Cysteine proteinases	F: Peptidase_C9	ECOD (1.6)
C	EUF08561	e4guaC6	A: a+b complex topology	X: nsP3 zinc-binding domain (From Topology)	H: nsP3 zinc-binding domain (From Topology)	T: nsP3 zinc-binding domain	F: EUF08561	ECOD (1.6)
C	FtsJ_1	e4guaC1	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: FtsJ_1	ECOD (1.6)
C	Macro_3	e4guaC7	A: a/b three-layered sandwiches	X: Macro domain-like (From Topology)	H: Macro domain-like (From Topology)	T: Macro domain-like	F: Macro_3	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.90.70.110	Alpha Beta	Alpha-Beta Complex	Cathepsin B	Chain A	CATH (4.3.0)
A	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
A	3.40.220.10	Alpha Beta	3-Layer(aba) Sandwich	Leucine Aminopeptidase, subunit E	domain 1	CATH (4.3.0)
B	3.90.70.110	Alpha Beta	Alpha-Beta Complex	Cathepsin B	Chain A	CATH (4.3.0)
B	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
B	3.40.220.10	Alpha Beta	3-Layer(aba) Sandwich	Leucine Aminopeptidase, subunit E	domain 1	CATH (4.3.0)
C	3.90.70.110	Alpha Beta	Alpha-Beta Complex	Cathepsin B	Chain A	CATH (4.3.0)
C	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
C	3.40.220.10	Alpha Beta	3-Layer(aba) Sandwich	Leucine Aminopeptidase, subunit E	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF01707	Peptidase family C9 (Peptidase_C9)	Peptidase family C9	-	Family
A, B, C	PF01661	Macro domain (Macro)	Macro domain	The Macro or A1pp domain is a module of about 180 amino acids which can bind ADP-ribose (an NAD metabolite) or related ligands. Binding to ADP-ribose could be either covalent or non-covalent [1]: in certain cases it is believed to bind non-covalently ...	The Macro or A1pp domain is a module of about 180 amino acids which can bind ADP-ribose (an NAD metabolite) or related ligands. Binding to ADP-ribose could be either covalent or non-covalent [1]: in certain cases it is believed to bind non-covalently [2]; while in other cases (such as Aprataxin) it appears to bind both non-covalently through a zinc finger motif, and covalently through a separate region of the protein [3]. This domain is found in a number of otherwise unrelated proteins. It is found at the C-terminus of the macro-H2A histone protein 4 and also in the non-structural proteins of several types of ssRNA viruses such as NSP3 from alpha-viruses and coronaviruses. This domain is also found on its own in a family of proteins from bacteria, archaebacteria and eukaryotes. The 3D structure of the SARS-CoV Macro domain has a mixed alpha/beta fold consisting of a central seven-stranded twisted mixed beta sheet sandwiched between two alpha helices on one face, and three on the other. The final alpha-helix, located on the edge of the central beta-sheet, forms the C terminus of the protein [4]. The crystal structure of AF1521 (a Macro domain-only protein from Archaeoglobus fulgidus) has also been reported and compared with other Macro domain containing proteins. Several Macro domain only proteins are shorter than AF1521, and appear to lack either the first strand of the beta-sheet or the C-terminal helix 5. Well conserved residues form a hydrophobic cleft and cluster around the AF1521-ADP-ribose binding site [5].	Domain
A, B, C	PF20852	Non-structural protein 3, zinc-binding domain (nsP3_ZBD)	Non-structural protein 3, zinc-binding domain	This domain is found in Non-structural protein 3 (nsP3) from Chikungunya virus (CHIKV) and similar proteins from Alphavirus. The function of nsP3, one of the four products of the polyprotein P1234, is still unknown. This protein consists of three dom ...	This domain is found in Non-structural protein 3 (nsP3) from Chikungunya virus (CHIKV) and similar proteins from Alphavirus. The function of nsP3, one of the four products of the polyprotein P1234, is still unknown. This protein consists of three domains: an amino-terminal Macro domain (Pfam:PF01661), a central zinc- binding domain (ZBD, this entry), and a hypervariable-sequence carboxyl terminus. ZBD is essential for viral replication. It has two cysteines in a loop between alpha-helices and the other two cysteines at the end of two parallel beta-strands, which represent a structural scaffold for zinc-ion coordination [1,2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	Non-structural polyprotein	5'-3' RNA polymerase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid RNA polymerase activity transferase activity RNA-dependent RNA polymerase activity nucleotidyltransferase activity catalytic activity transferase activity, transferring phosphorus-containing groups RNA methyltransferase activity transferase activity, transferring one-carbon groups methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity mRNA methyltransferase activity 5'-3' RNA polymerase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid RNA polymerase activity transferase activity RNA-dependent RNA polymerase activity nucleotidyltransferase activity catalytic activity transferase activity, transferring phosphorus-containing groups RNA methyltransferase activity transferase activity, transferring one-carbon groups methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity mRNA methyltransferase activity cysteine-type peptidase activity peptidase activity catalytic activity, acting on a protein hydrolase activity RNA binding binding organic cyclic compound binding nucleic acid binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding adenyl ribonucleotide binding adenyl nucleotide binding ATP binding phosphoric ester hydrolase activity hydrolase activity, acting on ester bonds polynucleotide 5'-phosphatase activity phosphatase activity pyrophosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity, acting on acid anhydrides mRNA 5'-triphosphate monophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity ATP-dependent activity poly(A) RNA polymerase activity adenylyltransferase activity helicase activity RNA helicase activity ATP-dependent activity, acting on RNA cation binding metal ion binding	symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms symbiont-mediated suppression of host JAK-STAT cascade biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated suppression of host signal transduction pathway symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT activity symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity modulation by symbiont of host process cellular process symbiont-mediated perturbation of host signal transduction pathway modulation of process of another organism cellular biosynthetic process RNA metabolic process symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms symbiont-mediated suppression of host JAK-STAT cascade biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated suppression of host signal transduction pathway symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT activity symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity modulation by symbiont of host process cellular process symbiont-mediated perturbation of host signal transduction pathway modulation of process of another organism cellular biosynthetic process RNA metabolic process viral life cycle RNA biosynthetic process nucleobase-containing compound metabolic process nucleic acid biosynthetic process organic substance metabolic process viral RNA genome replication viral process organic substance biosynthetic process positive stranded viral RNA replication cellular metabolic process primary metabolic process nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process organic cyclic compound metabolic process viral genome replication biosynthetic process organic cyclic compound biosynthetic process metabolic process macromolecule metabolic process gene expression DNA-templated transcription symbiont-mediated suppression of host transcription symbiont-mediated suppression of host gene expression symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity symbiont-mediated perturbation of host gene expression symbiont-mediated perturbation of host transcription symbiont-mediated suppression of host type I interferon-mediated signaling pathway symbiont-mediated suppression of host interferon-mediated signaling pathway 7-methylguanosine RNA capping RNA capping mRNA processing RNA processing 7-methylguanosine mRNA capping mRNA metabolic process RNA 5'-end processing organonitrogen compound metabolic process proteolysis protein metabolic process	membrane cellular anatomical entity host cell plasma membrane host cellular component host cell part host cell membrane host intracellular membrane-bounded organelle host intracellular part host cell cytoplasmic vesicle membrane host cell cytoplasm part host intracellular organelle host cell cytoplasmic vesicle host cell cytoplasm host intracellular region membrane cellular anatomical entity host cell plasma membrane host cellular component host cell part host cell membrane host intracellular membrane-bounded organelle host intracellular part host cell cytoplasmic vesicle membrane host cell cytoplasm part host intracellular organelle host cell cytoplasmic vesicle host cell cytoplasm host intracellular region host cell filopodium host cell projection host cell nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR047311	RNA-dependent RNA polymerase, togavirus	Domain
A, B, C	IPR048891	Non-structural protein 3, zinc-binding domain	Domain
A, B, C	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B, C	IPR002589	Macro domain	Domain
A, B, C	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily
A, B, C	IPR002620	Alphavirus nsp2 protease domain	Domain
A, B, C	IPR043502	DNA/RNA polymerase superfamily	Homologous Superfamily
A, B, C	IPR001788	RNA-dependent RNA polymerase, alsuviricetes	Domain
A, B, C	IPR002588	Alphavirus-like methyltransferase domain	Domain
A, B, C	IPR043472	Macro domain-like	Homologous Superfamily
A, B, C	IPR044936	Alphavirus nsP2 protease domain superfamily	Homologous Superfamily
A, B, C	IPR049329	Tomato mosaic virus helicase, N-terminal domain	Domain
A, B, C	IPR044371	Non-structural protein 3, X-domain-like	Domain
A, B, C	IPR027351	(+) RNA virus helicase core domain	Domain
A, B, C	IPR007094	RNA-directed RNA polymerase, catalytic domain	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.