3Q0A

X-ray crystal structure of the transcription initiation complex of the N4 mini-vRNAP with P2 promoter: Mismatch complex

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF21894	e3q0aA2	A: alpha arrays	X: helical bundle in Bacillus stearothermophilus-like DNA polymerase I (From Topology)	H: helical bundle in Bacillus stearothermophilus-like DNA polymerase I (From Topology)	T: helical bundle in Bacillus stearothermophilus-like DNA polymerase I	F: PF21894	ECOD (1.6)
A	PF21624	e3q0aA5	A: alpha arrays	X: inserted helical bundle subdomain in T7 RNA polymerase (From Topology)	H: inserted helical bundle subdomain in T7 RNA polymerase (From Topology)	T: inserted helical bundle subdomain in T7 RNA polymerase	F: PF21624	ECOD (1.6)
A	PF21867	e3q0aA3	A: alpha bundles	X: fingers domain in bacteriophage RB69-like DNA polymerase I (From Topology)	H: fingers domain in bacteriophage RB69-like DNA polymerase I (From Topology)	T: fingers domain in bacteriophage RB69-like DNA polymerase I	F: PF21867	ECOD (1.6)
A	PF21769	e3q0aA1	A: a+b two layers	X: Alpha-beta plaits	H: Adenylyl and guanylyl cyclase catalytic domain-like	T: Adenylyl and guanylyl cyclase catalytic domain-like	F: PF21769	ECOD (1.6)
C [auth B]	PF21894	e3q0aB1	A: alpha arrays	X: helical bundle in Bacillus stearothermophilus-like DNA polymerase I (From Topology)	H: helical bundle in Bacillus stearothermophilus-like DNA polymerase I (From Topology)	T: helical bundle in Bacillus stearothermophilus-like DNA polymerase I	F: PF21894	ECOD (1.6)
C [auth B]	PF21624	e3q0aB3	A: alpha arrays	X: inserted helical bundle subdomain in T7 RNA polymerase (From Topology)	H: inserted helical bundle subdomain in T7 RNA polymerase (From Topology)	T: inserted helical bundle subdomain in T7 RNA polymerase	F: PF21624	ECOD (1.6)
C [auth B]	PF21867	e3q0aB4	A: alpha bundles	X: fingers domain in bacteriophage RB69-like DNA polymerase I (From Topology)	H: fingers domain in bacteriophage RB69-like DNA polymerase I (From Topology)	T: fingers domain in bacteriophage RB69-like DNA polymerase I	F: PF21867	ECOD (1.6)
C [auth B]	PF21769	e3q0aB5	A: a+b two layers	X: Alpha-beta plaits	H: Adenylyl and guanylyl cyclase catalytic domain-like	T: Adenylyl and guanylyl cyclase catalytic domain-like	F: PF21769	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.70.2440	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits		CATH (4.3.0)
A	6.10.140.1360	Special	Helix non-globular	Helix Hairpins		CATH (4.3.0)
A	6.10.140.1370	Special	Helix non-globular	Helix Hairpins		CATH (4.3.0)
A	6.10.250.1860	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
A	3.30.750.220	Alpha Beta	2-Layer Sandwich	Transcription Regulator spoIIAA		CATH (4.3.0)
A	1.20.140.110	Mainly Alpha	Up-down Bundle	Butyryl-CoA Dehydrogenase, subunit A	domain 3	CATH (4.3.0)
C [auth B]	3.30.70.2440	Alpha Beta	2-Layer Sandwich	Alpha-Beta Plaits		CATH (4.3.0)
C [auth B]	6.10.140.1360	Special	Helix non-globular	Helix Hairpins		CATH (4.3.0)
C [auth B]	6.10.140.1370	Special	Helix non-globular	Helix Hairpins		CATH (4.3.0)
C [auth B]	6.10.250.1860	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
C [auth B]	3.30.750.220	Alpha Beta	2-Layer Sandwich	Transcription Regulator spoIIAA		CATH (4.3.0)
C [auth B]	1.20.140.110	Mainly Alpha	Up-down Bundle	Butyryl-CoA Dehydrogenase, subunit A	domain 3	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C [auth B]	PF21769	Virion DNA-directed RNA polymerase domain (vRNAP_dom)	Virion DNA-directed RNA polymerase domain	This domain is found in Virion DNA-directed RNA polymerase from Bacteriophage N4 (vRNAP), responsible for the transcription of the early region of the double-stranded linear DNA genome of the lytic coliphage. This domain adopts a mostly alpha-helical ...	This domain is found in Virion DNA-directed RNA polymerase from Bacteriophage N4 (vRNAP), responsible for the transcription of the early region of the double-stranded linear DNA genome of the lytic coliphage. This domain adopts a mostly alpha-helical configuration [1-3].	Domain
A, C [auth B]	PF21624	Virion DNA-directed RNA polymerase, plug insertion (vRNAP_plug)	Virion DNA-directed RNA polymerase, plug insertion	This domain is found in Virion DNA-directed RNA polymerase from Bacteriophage N4 (vRNAP), responsible for the transcription of the early region of the double-stranded linear DNA genome of the lytic coliphage. This entry represents a short insertion t ...	This domain is found in Virion DNA-directed RNA polymerase from Bacteriophage N4 (vRNAP), responsible for the transcription of the early region of the double-stranded linear DNA genome of the lytic coliphage. This entry represents a short insertion that forms a globular module named plug, which blocks the activity of the protein [1-3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C [auth B]	Virion RNA polymerase	binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding guanyl nucleotide binding GTP binding guanyl ribonucleotide binding DNA-directed 5'-3' RNA polymerase activity 5'-3' RNA polymerase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid RNA polymerase activity transferase activity nucleotidyltransferase activity catalytic activity transferase activity, transferring phosphorus-containing groups cation binding metal ion binding	-	virion component transferase complex, transferring phosphorus-containing groups protein-containing complex catalytic complex RNA polymerase complex DNA-directed RNA polymerase complex transferase complex intracellular protein-containing complex
B [auth C], D	DNA (5'-D(TPGPCPCPTPCPCPCPAPGPGPCPAGPTPCPAPAPAPAPGPAPAPGPCPGPGPAPGPCPTPTPCPTPTPC)-3')	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C [auth B]	IPR049432	Virion DNA-directed RNA polymerase domain	Domain
A, C [auth B]	IPR049433	Virion DNA-directed RNA polymerase, plug insertion domain	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.