3L4Q

Structural insights into phosphoinositide 3-kinase activation by the influenza A virus NS1 protein

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
C [auth B]	d3l4qb_	Alpha and beta proteins (a+b)	Ns1 effector domain-like	Ns1 effector domain-like	Ns1 effector domain-like	automated matches	(Influenza A virus (A/Puerto Rico/8/1934(H1N1)) ) [TaxId: 211044 ],	SCOPe (2.08)
A	d3l4qa_	Alpha and beta proteins (a+b)	Ns1 effector domain-like	Ns1 effector domain-like	Ns1 effector domain-like	automated matches	(Influenza A virus (A/Puerto Rico/8/1934(H1N1)) ) [TaxId: 211044 ],	SCOPe (2.08)
B [auth C]	d3l4qc_	Coiled coil proteins	Antiparallel coiled-coil	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 (iSH2) domain-like	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain-like	automated matches	bovine (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)
D	d3l4qd_	Coiled coil proteins	Antiparallel coiled-coil	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 (iSH2) domain-like	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain-like	automated matches	bovine (Bos taurus ) [TaxId: 9913 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B [auth C]	SCOP2 Family	PI3K inter-SH2 coiled-coil domain	8045617	4004569	SCOP2 (2022-06-29)
B [auth C]	SCOP2 Superfamily	PI3K inter-SH2 coiled-coil domain	8052381	3002111	SCOP2 (2022-06-29)
D	SCOP2B Superfamily	PI3K inter-SH2 coiled-coil domain	8052381	3002111	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
C [auth B]	Flu_NS1_C	e3l4qB1	A: mixed a+b and a/b	X: Ns1 effector domain-like (From Topology)	H: Ns1 effector domain-like (From Topology)	T: Ns1 effector domain-like	F: Flu_NS1_C	ECOD (1.6)
A	Flu_NS1_C	e3l4qA1	A: mixed a+b and a/b	X: Ns1 effector domain-like (From Topology)	H: Ns1 effector domain-like (From Topology)	T: Ns1 effector domain-like	F: Flu_NS1_C	ECOD (1.6)
B [auth C]	PI3K_P85_iSH2	e3l4qC1	A: alpha bundles	X: YscO-like	H: Phosphatidylinositol 3-kinase regulatory subunit inter-SH2 domain (From Topology)	T: Phosphatidylinositol 3-kinase regulatory subunit inter-SH2 domain	F: PI3K_P85_iSH2	ECOD (1.6)
D	PI3K_P85_iSH2	e3l4qD1	A: alpha bundles	X: YscO-like	H: Phosphatidylinositol 3-kinase regulatory subunit inter-SH2 domain (From Topology)	T: Phosphatidylinositol 3-kinase regulatory subunit inter-SH2 domain	F: PI3K_P85_iSH2	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
C [auth B]	3.30.420.330	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
A	3.30.420.330	Alpha Beta	2-Layer Sandwich	Nucleotidyltransferase	domain 5	CATH (4.3.0)
B [auth C]	1.10.287.1490	Mainly Alpha	Orthogonal Bundle	Helix Hairpins		CATH (4.3.0)
D	1.10.287.1490	Mainly Alpha	Orthogonal Bundle	Helix Hairpins		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, C [auth B]	PF00600	Influenza non-structural protein (NS1) (Flu_NS1)	Influenza non-structural protein (NS1)	-	Family
B [auth C], D	PF16454	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain (PI3K_P85_iSH2)	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain	This domain is found between the two SH2 domains in phosphatidylinositol 3-kinase regulatory subunit P85. It forms a complex with the adaptor-binding domain of phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, C [auth B]	Non-structural protein 1	enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase regulator activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity kinase regulator activity RNA binding binding organic cyclic compound binding nucleic acid binding identical protein binding enzyme regulator activity protein kinase inhibitor activity molecular function inhibitor activity molecular function regulator activity enzyme inhibitor activity protein kinase regulator activity protein serine/threonine kinase inhibitor activity kinase inhibitor activity kinase regulator activity RNA binding binding organic cyclic compound binding nucleic acid binding identical protein binding protein binding	symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms symbiont-mediated suppression of host PKR/eIFalpha signaling symbiont-mediated perturbation of host translation biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated suppression of host signal transduction pathway symbiont-mediated suppression of host-directed shutoff of host translation modulation by symbiont of host process symbiont-mediated perturbation of host gene expression cellular process symbiont-mediated perturbation of host signal transduction pathway modulation of process of another organism symbiont-mediated suppression of host type I interferon-mediated signaling pathway symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms symbiont-mediated suppression of host PKR/eIFalpha signaling symbiont-mediated perturbation of host translation biological process involved in interaction with host biological process involved in symbiotic interaction symbiont-mediated suppression of host signal transduction pathway symbiont-mediated suppression of host-directed shutoff of host translation modulation by symbiont of host process symbiont-mediated perturbation of host gene expression cellular process symbiont-mediated perturbation of host signal transduction pathway modulation of process of another organism symbiont-mediated suppression of host type I interferon-mediated signaling pathway symbiont-mediated suppression of host interferon-mediated signaling pathway symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity response to stimulus symbiont-mediated suppression of host pathogen-associated molecular pattern receptor signaling pathway response to biotic stimulus symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response response to host immune response symbiont-mediated suppression of host defenses symbiont-mediated perturbation of host innate immune response response to host response to external biotic stimulus symbiont-mediated perturbation of host defense response response to host defenses response to other organism symbiont-mediated perturbation of host immune response response to defenses of other organism symbiont-mediated perturbation of host defenses response to external stimulus symbiont-mediated suppression of cytoplasmic pattern recognition receptor signaling pathway symbiont-mediated suppression of host mRNA processing symbiont-mediated suppression of host gene expression	host intracellular membrane-bounded organelle host cellular component host intracellular part host cell part host intracellular region cellular anatomical entity host cell nucleus host intracellular organelle host cell cytoplasm
B [auth C], D	Phosphatidylinositol 3-kinase regulatory subunit beta	enzyme regulator activity molecular function regulator activity 1-phosphatidylinositol-3-kinase regulator activity kinase regulator activity	cellular homeostasis chemical homeostasis homeostatic process glucose homeostasis carbohydrate homeostasis intracellular chemical homeostasis intracellular glucose homeostasis cellular response to stimulus cellular response to nitrogen compound response to organic substance regulation of cellular process response to stimulus signaling response to peptide hormone cellular homeostasis chemical homeostasis homeostatic process glucose homeostasis carbohydrate homeostasis intracellular chemical homeostasis intracellular glucose homeostasis cellular response to stimulus cellular response to nitrogen compound response to organic substance regulation of cellular process response to stimulus signaling response to peptide hormone insulin receptor signaling pathway cell surface receptor protein tyrosine kinase signaling pathway cell communication cellular response to endogenous stimulus response to hormone cellular response to organic substance signal transduction enzyme-linked receptor protein signaling pathway cellular response to hormone stimulus regulation of biological process cell surface receptor signaling pathway response to endogenous stimulus response to organonitrogen compound biological regulation response to oxygen-containing compound cellular response to peptide hormone stimulus response to nitrogen compound cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to organonitrogen compound response to chemical response to insulin cellular process cellular response to insulin stimulus intracellular signal transduction intracellular signaling cassette phosphatidylinositol 3-kinase/protein kinase B signal transduction cellular biosynthetic process glycerophospholipid metabolic process lipid biosynthetic process phospholipid metabolic process organic substance metabolic process organic substance biosynthetic process phosphate-containing compound metabolic process phosphorus metabolic process phosphatidylinositol metabolic process glycerophospholipid biosynthetic process cellular metabolic process primary metabolic process phosphatidylinositol phosphate biosynthetic process phospholipid biosynthetic process phosphatidylinositol biosynthetic process biosynthetic process glycerolipid biosynthetic process organophosphate metabolic process organophosphate biosynthetic process cellular lipid metabolic process metabolic process glycerolipid metabolic process lipid metabolic process protein localization establishment of protein localization cellular localization nitrogen compound transport transport organic substance transport establishment of localization localization protein transport cellular macromolecule localization macromolecule localization catabolic process autophagy regulation of cellular metabolic process regulation of catabolic process regulation of autophagy cellular catabolic process regulation of cellular catabolic process regulation of metabolic process process utilizing autophagic mechanism positive regulation of macromolecule biosynthetic process regulation of primary metabolic process nucleobase-containing compound metabolic process positive regulation of metabolic process DNA-templated transcription regulation of RNA metabolic process transcription by RNA polymerase II positive regulation of macromolecule metabolic process regulation of nucleobase-containing compound metabolic process regulation of transcription by RNA polymerase II positive regulation of nucleobase-containing compound metabolic process positive regulation of transcription by RNA polymerase II positive regulation of RNA metabolic process organic cyclic compound metabolic process regulation of gene expression organic cyclic compound biosynthetic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of DNA-templated transcription positive regulation of DNA-templated transcription positive regulation of biological process RNA metabolic process positive regulation of cellular metabolic process RNA biosynthetic process nucleic acid biosynthetic process positive regulation of biosynthetic process positive regulation of cellular process regulation of RNA biosynthetic process regulation of biosynthetic process regulation of macromolecule metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process regulation of cellular biosynthetic process positive regulation of RNA biosynthetic process positive regulation of cellular biosynthetic process regulation of localization nucleocytoplasmic transport nuclear transport positive regulation of establishment of protein localization import into nucleus establishment of localization in cell regulation of intracellular transport positive regulation of protein localization to nucleus positive regulation of protein import into nucleus intracellular transport regulation of nucleocytoplasmic transport protein localization to nucleus establishment of protein localization to organelle positive regulation of protein localization regulation of intracellular protein transport positive regulation of intracellular protein transport intracellular protein transport regulation of protein localization positive regulation of nucleocytoplasmic transport regulation of protein localization to nucleus positive regulation of protein transport regulation of protein import into nucleus protein import into nucleus positive regulation of intracellular transport regulation of transport regulation of establishment of protein localization regulation of protein transport positive regulation of transport regulation of cellular localization protein localization to organelle cellular response to stress response to stress response to endoplasmic reticulum stress	phosphatidylinositol 3-kinase complex transferase complex, transferring phosphorus-containing groups protein-containing complex catalytic complex membrane membrane protein complex cellular anatomical entity transferase complex extrinsic component of membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle nucleus phosphatidylinositol 3-kinase complex transferase complex, transferring phosphorus-containing groups protein-containing complex catalytic complex membrane membrane protein complex cellular anatomical entity transferase complex extrinsic component of membrane membrane-bounded organelle intracellular organelle intracellular anatomical structure organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, C [auth B]	IPR038064	Influenza A virus NS1, effector domain-like superfamily	Homologous Superfamily
A, C [auth B]	IPR009068	uS15/NS1, RNA-binding domain superfamily	Homologous Superfamily
A, C [auth B]	IPR000256	Influenza A virus NS1 protein	Family
A, C [auth B]	IPR004208	Influenza non-structural protein (NS1)	Family
B [auth C], D	IPR000198	Rho GTPase-activating protein domain	Domain
B [auth C], D	IPR032498	PI3K regulatory subunit p85-related , inter-SH2 domain	Domain
B [auth C], D	IPR036860	SH2 domain superfamily	Homologous Superfamily
B [auth C], D	IPR000980	SH2 domain	Domain
B [auth C], D	IPR035586	Phosphatidylinositol 3-kinase regulatory subunit beta, SH3 domain	Domain
B [auth C], D	IPR001452	SH3 domain	Domain
B [auth C], D	IPR036028	SH3-like domain superfamily	Homologous Superfamily
B [auth C], D	IPR035022	PI3K p85 subunit, N-terminal SH2 domain	Domain
B [auth C], D	IPR008936	Rho GTPase activation protein	Homologous Superfamily
B [auth C], D	IPR035020	PI3K p85 subunit, C-terminal SH2 domain	Domain

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.