3GZF

Structure of the C-terminal domain of nsp4 from Feline Coronavirus

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Coronavirus NSP4 C-terminal domain-like	8045407	4004459	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Coronavirus NSP4 C-terminal domain-like	8090553	3002444	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Coronavirus NSP4 C-terminal domain-like	8090553	3002444	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Coronavirus NSP4 C-terminal domain-like	8090553	3002444	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Coronavirus NSP4 C-terminal domain-like	8090553	3002444	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Coronavirus NSP4 C-terminal domain-like	8090553	3002444	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF16348	e3gzfA1	A: a+b two layers	X: C-terminal domain of nonstructural protein nsp4 (From Topology)	H: C-terminal domain of nonstructural protein nsp4 (From Topology)	T: C-terminal domain of nonstructural protein nsp4	F: PF16348	ECOD (1.6)
B	PF16348	e3gzfB1	A: a+b two layers	X: C-terminal domain of nonstructural protein nsp4 (From Topology)	H: C-terminal domain of nonstructural protein nsp4 (From Topology)	T: C-terminal domain of nonstructural protein nsp4	F: PF16348	ECOD (1.6)
C	PF16348	e3gzfC1	A: a+b two layers	X: C-terminal domain of nonstructural protein nsp4 (From Topology)	H: C-terminal domain of nonstructural protein nsp4 (From Topology)	T: C-terminal domain of nonstructural protein nsp4	F: PF16348	ECOD (1.6)
D	PF16348	e3gzfD1	A: a+b two layers	X: C-terminal domain of nonstructural protein nsp4 (From Topology)	H: C-terminal domain of nonstructural protein nsp4 (From Topology)	T: C-terminal domain of nonstructural protein nsp4	F: PF16348	ECOD (1.6)
E	PF16348	e3gzfE1	A: a+b two layers	X: C-terminal domain of nonstructural protein nsp4 (From Topology)	H: C-terminal domain of nonstructural protein nsp4 (From Topology)	T: C-terminal domain of nonstructural protein nsp4	F: PF16348	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.150.420	Mainly Alpha	Orthogonal Bundle	DNA polymerase	domain 1	CATH (4.3.0)
B	1.10.150.420	Mainly Alpha	Orthogonal Bundle	DNA polymerase	domain 1	CATH (4.3.0)
C	1.10.150.420	Mainly Alpha	Orthogonal Bundle	DNA polymerase	domain 1	CATH (4.3.0)
D	1.10.150.420	Mainly Alpha	Orthogonal Bundle	DNA polymerase	domain 1	CATH (4.3.0)
E	1.10.150.420	Mainly Alpha	Orthogonal Bundle	DNA polymerase	domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E	PF16348	Coronavirus replicase NSP4, C-terminal (CoV_NSP4_C)	Coronavirus replicase NSP4, C-terminal	This is the C-terminal domain of the coronavirus nonstructural protein 4 (NSP4). NSP4 is encoded by ORF1a/1ab and proteolytically released from the pp1a/1ab polyprotein. It is a membrane-spanning protein which is thought to anchor the viral replicati ...	This is the C-terminal domain of the coronavirus nonstructural protein 4 (NSP4). NSP4 is encoded by ORF1a/1ab and proteolytically released from the pp1a/1ab polyprotein. It is a membrane-spanning protein which is thought to anchor the viral replication-transcription complex (RTC) to modified endoplasmic reticulum membranes. This predominantly alpha-helical domain may be involved in protein-protein interactions [1]. It has been shown that in Betacoronavirus, the coexpression of NSP3 and NSP4 results in a membrane rearrangement to induce double-membrane vesicles (DMVs) and convoluted membranes (CMs), playing a critical role in SARS-CoV replication. There are two well conserved amino acid residues (H120 and F121) in NSP4 among Betacoronavirus, essential for membrane rearrangements during interaction with NSP3 [2,3].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E	Replicase polyprotein 1ab	peptidase activity omega peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity 3'-5'-RNA exonuclease activity catalytic activity, acting on RNA RNA exonuclease activity, producing 5'-phosphomonoesters catalytic activity, acting on a nucleic acid 3'-5' exonuclease activity RNA exonuclease activity RNA nuclease activity exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters nuclease activity peptidase activity omega peptidase activity catalytic activity, acting on a protein hydrolase activity catalytic activity 3'-5'-RNA exonuclease activity catalytic activity, acting on RNA RNA exonuclease activity, producing 5'-phosphomonoesters catalytic activity, acting on a nucleic acid 3'-5' exonuclease activity RNA exonuclease activity RNA nuclease activity exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters nuclease activity hydrolase activity, acting on ester bonds exonuclease activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding organic cyclic compound binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding helicase activity RNA helicase activity ATP-dependent activity, acting on RNA ATP-dependent activity endonuclease activity RNA methyltransferase activity O-methyltransferase activity transferase activity, transferring one-carbon groups mRNA (nucleoside-2'-O-)-methyltransferase activity transferase activity S-adenosylmethionine-dependent methyltransferase activity methyltransferase activity mRNA methyltransferase activity 5'-3' RNA polymerase activity RNA polymerase activity RNA-dependent RNA polymerase activity nucleotidyltransferase activity transferase activity, transferring phosphorus-containing groups mRNA 5'-cap (guanine-N7-)-methyltransferase activity N-methyltransferase activity cysteine-type peptidase activity cysteine-type endopeptidase activity endopeptidase activity lyase activity RNA binding nucleic acid binding ubiquitin-like protein peptidase activity cysteine-type deubiquitinase activity deubiquitinase activity pyrophosphatase activity ATP hydrolysis activity ribonucleoside triphosphate phosphatase activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity, acting on acid anhydrides cation binding transition metal ion binding zinc ion binding metal ion binding	viral gene expression viral protein processing viral process cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription viral gene expression viral protein processing viral process cellular biosynthetic process cellular metabolic process RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription organic cyclic compound metabolic process nucleic acid biosynthetic process organic substance metabolic process biosynthetic process organic cyclic compound biosynthetic process organic substance biosynthetic process metabolic process cellular process macromolecule metabolic process symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms response to stimulus symbiont-mediated suppression of host pathogen-associated molecular pattern receptor signaling pathway response to biotic stimulus biological process involved in symbiotic interaction symbiont-mediated suppression of host immune response symbiont-mediated suppression of host innate immune response response to host immune response symbiont-mediated suppression of host defenses symbiont-mediated perturbation of host innate immune response response to host response to external biotic stimulus symbiont-mediated perturbation of host signal transduction pathway modulation of process of another organism symbiont-mediated perturbation of host defense response response to host defenses response to other organism symbiont-mediated perturbation of host immune response response to defenses of other organism symbiont-mediated perturbation of host defenses response to external stimulus biological process involved in interaction with host symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity symbiont-mediated suppression of host signal transduction pathway modulation by symbiont of host process symbiont-mediated suppression of cytoplasmic pattern recognition receptor signaling pathway catabolic process regulation of cellular process autophagy positive regulation of biological process modulation by virus of host process positive regulation of cellular metabolic process regulation of autophagy cellular catabolic process positive regulation of metabolic process induction by virus of host autophagy process utilizing autophagic mechanism positive regulation of cellular process modulation by virus of host cellular process positive regulation of autophagy regulation of biological process modulation by virus of host autophagy biological regulation regulation of cellular metabolic process regulation of catabolic process symbiont-mediated perturbation of host autophagy positive regulation of catabolic process regulation of cellular catabolic process positive regulation of cellular catabolic process regulation of metabolic process symbiont-mediated perturbation of host ubiquitin-like protein modification viral genome replication viral life cycle organonitrogen compound metabolic process proteolysis protein metabolic process	membrane cellular anatomical entity host intracellular membrane-bounded organelle host cellular component host intracellular part host cell endoplasmic reticulum-Golgi intermediate compartment host cell cytoplasm host cell part host intracellular region host cell cytoplasm part host intracellular organelle host cell perinuclear region of cytoplasm host cell membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E	IPR042515	Non-structural protein NSP15, N-terminal domain superfamily, coronavirus	Homologous Superfamily
A, B, C, D, E	IPR048673	Nonstructural protein 13, stalk domain, coronavirus	Domain
A, B, C, D, E	IPR046435	NSP14, guanine-N7-methyltransferase domain, coronavirus	Domain
A, B, C, D, E	IPR043174	Non-structural protein NSP15, middle domain superfamily	Homologous Superfamily
A, B, C, D, E	IPR009469	RNA-dependent RNA polymerase, N-terminal, coronavirus	Domain
A, B, C, D, E	IPR043611	Coronavirus replicase NSP3, C-terminal	Domain
A, B, C, D, E	IPR038123	Non-structural protein NSP4, C-terminal superfamily, coronavirus	Homologous Superfamily
A, B, C, D, E	IPR032039	Alphacoronavirus nsp1	Domain
A, B, C, D, E	IPR047566	Coronavirus replicase NSP3, Y domain	Domain
A, B, C, D, E	IPR043178	Papain-like protease, thumb domain superfamily, coronavirus	Homologous Superfamily
A, B, C, D, E	IPR046441	RNA-dependent RNA polymerase, coronavirus	Domain
A, B, C, D, E	IPR048672	Nonstructural protein 13, zinc-binding domain, coronavirus	Domain
A, B, C, D, E	IPR043612	Non-structural protein NSP4, N-terminal, coronavirus	Domain
A, B, C, D, E	IPR038634	Alphacoronavirus nsp1 domain superfamily	Homologous Superfamily
A, B, C, D, E	IPR044863	Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain	Domain
A, B, C, D, E	IPR044343	Nonstructural protein 13, 1B domain, coronavirus	Domain
A, B, C, D, E	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B, C, D, E	IPR043608	Nonstructural protein 15, middle domain, coronavirus	Domain
A, B, C, D, E	IPR044353	NSP3, second ubiquitin-like (Ubl) domain, coronavirus	Domain
A, B, C, D, E	IPR037227	Endoribonuclease EndoU-like	Homologous Superfamily
A, B, C, D, E	IPR027352	Nonstructural protein 13, zinc-binding domain, coronavirus-like	Domain
A, B, C, D, E	IPR009003	Peptidase S1, PA clan	Homologous Superfamily
A, B, C, D, E	IPR013016	Peptidase C16, coronavirus	Domain
A, B, C, D, E	IPR037204	Non-structural protein NSP7 superfamily, coronavirus	Homologous Superfamily
A, B, C, D, E	IPR047912	Non-structural protein 13, helicase domain, alphacoronavirus	Domain
A, B, C, D, E	IPR009466	Non-structural protein 14, coronavirus	Domain
A, B, C, D, E	IPR044357	NSP3, first ubiquitin-like (Ubl) domain, coronavirus	Domain
A, B, C, D, E	IPR046436	Nidovirus 3'-5' exoribonuclease domain	Domain
A, B, C, D, E	IPR046443	NSP1, globular domain, alpha/betacoronavirus	Domain
A, B, C, D, E	IPR047570	NSP12, interface domain, coronavirus	Domain
A, B, C, D, E	IPR044313	Nonstructural protein 14, alphacoronavirus	Domain
A, B, C, D, E	IPR043610	Non-structural protein 6, coronavirus	Domain
A, B, C, D, E	IPR027351	(+) RNA virus helicase core domain	Domain
A, B, C, D, E	IPR044322	Nonstructural protein 15, middle domain, alpha/betacoronavirus	Domain
A, B, C, D, E	IPR009461	Non-structural protein NSP16, coronavirus-like	Domain
A, B, C, D, E	IPR043477	Peptidase C30, domain 3, coronavirus	Homologous Superfamily
A, B, C, D, E	IPR018995	RNA synthesis protein NSP10, coronavirus	Domain
A, B, C, D, E	IPR043615	Nonstructural protein 2, N-terminal domain, coronavirus	Domain
A, B, C, D, E	IPR043177	Papain-like protease, N-terminal domain superfamily, coronavirus	Homologous Superfamily
A, B, C, D, E	IPR043606	Coronavirus replicase NSP15, N-terminal oligomerization	Domain
A, B, C, D, E	IPR044330	Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus	Domain
A, B, C, D, E	IPR001205	RNA-directed RNA polymerase, C-terminal domain	Domain
A, B, C, D, E	IPR044401	NSP15, NendoU domain, coronavirus	Domain
A, B, C, D, E	IPR044369	Non-structural protein 6, alphacoronavirus	Domain
A, B, C, D, E	IPR014822	Non-structural protein NSP9, coronavirus	Domain
A, B, C, D, E	IPR041679	DNA2/NAM7 helicase-like, C-terminal	Domain
A, B, C, D, E	IPR037230	Non-structural protein NSP8 superfamily, coronavirus	Homologous Superfamily
A, B, C, D, E	IPR044356	RNA-dependent RNA polymerase, alphacoronavirus	Domain
A, B, C, D, E	IPR046440	Arterivirus Nsp11 N-terminal/Coronavirus NSP15 middle domain	Domain
A, B, C, D, E	IPR014828	Non-structural protein NSP7, coronavirus	Domain
A, B, C, D, E	IPR032505	Non-structural protein NSP4, C-terminal, coronavirus	Domain
A, B, C, D, E	IPR014829	Non-structural protein NSP8, coronavirus	Domain
A, B, C, D, E	IPR046438	Nidovirus 2-O-methyltransferase	Domain
A, B, C, D, E	IPR036499	Non-structural protein NSP9 superfamily, coronavirus	Homologous Superfamily
A, B, C, D, E	IPR002589	Macro domain	Domain
A, B, C, D, E	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily
A, B, C, D, E	IPR043502	DNA/RNA polymerase superfamily	Homologous Superfamily
A, B, C, D, E	IPR008740	Peptidase C30, coronavirus	Domain
A, B, C, D, E	IPR036333	RNA synthesis protein NSP10 superfamily, coronavirus	Homologous Superfamily
A, B, C, D, E	IPR047573	Non-structural protein 2, middle domain, coronavirus	Domain
A, B, C, D, E	IPR044309	Non-structural protein 5, alphacoronavirus	Domain
A, B, C, D, E	IPR043472	Macro domain-like	Homologous Superfamily
A, B, C, D, E	IPR043609	NendoU domain, nidovirus	Domain
A, B, C, D, E	IPR044371	Non-structural protein 3, X-domain-like	Domain
A, B, C, D, E	IPR043613	Non-structural protein 2, C-terminal domain, coronavirus	Domain
A, B, C, D, E	IPR044385	Nonstructural protein 2, HCoV-229E-like	Domain
A, B, C, D, E	IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	Homologous Superfamily