2ZW3

Structure of the connexin-26 gap junction channel at 3.5 angstrom resolution

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Claudin-like	8092543	3002606	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Claudin-like	8092543	3002606	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Claudin-like	8092543	3002606	SCOP2B (2022-06-29)
D	SCOP2 Family	Connexin-like	8047374	4005615	SCOP2 (2022-06-29)
D	SCOP2 Superfamily	Claudin-like	8092543	3002606	SCOP2 (2022-06-29)
E	SCOP2B Superfamily	Claudin-like	8092543	3002606	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Claudin-like	8092543	3002606	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00029	e2zw3A1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
B	PF00029	e2zw3B1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
C	PF00029	e2zw3C1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
D	PF00029	e2zw3D1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
E	PF00029	e2zw3E1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)
F	PF00029	e2zw3F1	A: alpha bundles	X: Connexin 26 (From Topology)	H: Connexin 26 (From Topology)	T: Connexin 26	F: PF00029	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.20.1440.80	Mainly Alpha	Up-down Bundle	de novo design (two linked rop proteins)	Gap junction channel protein cysteine-rich domain	CATH (4.3.0)
B	1.20.1440.80	Mainly Alpha	Up-down Bundle	de novo design (two linked rop proteins)	Gap junction channel protein cysteine-rich domain	CATH (4.3.0)
C	1.20.1440.80	Mainly Alpha	Up-down Bundle	de novo design (two linked rop proteins)	Gap junction channel protein cysteine-rich domain	CATH (4.3.0)
D	1.20.1440.80	Mainly Alpha	Up-down Bundle	de novo design (two linked rop proteins)	Gap junction channel protein cysteine-rich domain	CATH (4.3.0)
E	1.20.1440.80	Mainly Alpha	Up-down Bundle	de novo design (two linked rop proteins)	Gap junction channel protein cysteine-rich domain	CATH (4.3.0)
F	1.20.1440.80	Mainly Alpha	Up-down Bundle	de novo design (two linked rop proteins)	Gap junction channel protein cysteine-rich domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F	PF00029	Connexin (Connexin)	Connexin	Connexin proteins form gap-junctions between cells. They carry four transmembrane regions, hence why this family now includes Connexin_CCC, which represented the second pair of TMs.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F	Gap junction beta-2 protein	cation binding ion binding binding calcium ion binding metal ion binding small molecule binding wide pore channel activity passive transmembrane transporter activity channel activity transporter activity gap junction channel activity involved in cell communication by electrical coupling gap junction channel activity transmembrane transporter activity identical protein binding cation binding ion binding binding calcium ion binding metal ion binding small molecule binding wide pore channel activity passive transmembrane transporter activity channel activity transporter activity gap junction channel activity involved in cell communication by electrical coupling gap junction channel activity transmembrane transporter activity identical protein binding protein binding	epididymis development developmental process multicellular organism development multicellular organismal process tube development anatomical structure development response to organic substance response to ketone response to lipid response to stimulus response to endogenous stimulus response to progesterone response to oxygen-containing compound response to hormone epididymis development developmental process multicellular organism development multicellular organismal process tube development anatomical structure development response to organic substance response to ketone response to lipid response to stimulus response to endogenous stimulus response to progesterone response to oxygen-containing compound response to hormone response to steroid hormone response to chemical response to organic cyclic compound response to stress response to ischemia ear development sensory organ development inner ear development animal organ development cellular response to stimulus cellular response to nitrogen compound response to organonitrogen compound response to peptide hormone cellular response to peptide hormone stimulus response to nitrogen compound response to glucagon cellular response to oxygen-containing compound cellular response to glucagon stimulus cellular response to endogenous stimulus cellular response to chemical stimulus cellular response to organonitrogen compound cellular process cellular response to organic substance cellular response to hormone stimulus regulation of biological process signaling biological regulation cell-cell signaling cell communication response to retinoic acid cellular response to ketone cellular response to steroid hormone stimulus cellular response to dexamethasone stimulus response to glucocorticoid cellular response to organic cyclic compound cellular response to glucocorticoid stimulus cellular response to corticosteroid stimulus response to corticosteroid cellular response to lipid response to dexamethasone response to oxidative stress cellular response to stress cellular response to oxidative stress cellular response to chemical stress transmembrane transport localization transport establishment of localization cellular component organization cellular component biogenesis cell junction organization cellular component assembly cell junction assembly gap junction assembly cellular component organization or biogenesis cell-cell junction assembly cell-cell junction organization response to human chorionic gonadotropin response to gonadotropin multi-multicellular organism process placenta development reproductive process maternal process involved in female pregnancy multicellular organismal reproductive process developmental process involved in reproduction tissue development maternal placenta development female pregnancy multi-organism reproductive process decidualization system process sensory perception nervous system process sensory perception of sound sensory perception of mechanical stimulus gap junction-mediated intercellular transport intercellular transport biological process involved in interspecies interaction between organisms response to other organism response to external stimulus response to lipopolysaccharide response to biotic stimulus response to bacterium response to external biotic stimulus response to molecule of bacterial origin response to estradiol	cell-cell junction protein-containing complex anchoring junction plasma membrane protein complex gap junction membrane plasma membrane membrane protein complex cellular anatomical entity cell periphery cell junction connexin complex glial cell projection cell projection cell-cell junction protein-containing complex anchoring junction plasma membrane protein complex gap junction membrane plasma membrane membrane protein complex cellular anatomical entity cell periphery cell junction connexin complex glial cell projection cell projection plasma membrane bounded cell projection astrocyte projection intracellular anatomical structure perinuclear region of cytoplasm cytoplasm cell body lateral plasma membrane membrane-bounded organelle intracellular organelle organelle endoplasmic reticulum-Golgi intermediate compartment intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F	IPR038359	Connexin, N-terminal domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F	IPR000500	Connexin	Family
A, B, C, D, E A, B, C, D, E, F	IPR019570	Gap junction protein, cysteine-rich domain	Domain
A, B, C, D, E A, B, C, D, E, F	IPR013092	Connexin, N-terminal	Domain
A, B, C, D, E A, B, C, D, E, F	IPR017990	Connexin, conserved site	Conserved Site
A, B, C, D, E A, B, C, D, E, F	IPR002268	Gap junction beta-2 protein (Cx26)	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D, E A, B, C, D, E, F	Pharos: P29033	Bart-Pumphrey syndrome palmoplantar keratoderma-deafness syndrome ichthyosis keratitis keratoderma hereditarium mutilans palmoplantar keratosis psoriasis ichthyosis, hystrix-like, with deafness nonsyndromic genetic deafness autosomal recessive nonsyndromic deafness 1A X-linked mixed deafness with perilymphatic gusher hidradenitis suppurativa autosomal dominant nonsyndromic deafness 3A porokeratotic eccrine ostial and dermal duct nevus skin cancer Bart-Pumphrey syndrome palmoplantar keratoderma-deafness syndrome ichthyosis keratitis keratoderma hereditarium mutilans palmoplantar keratosis psoriasis ichthyosis, hystrix-like, with deafness nonsyndromic genetic deafness autosomal recessive nonsyndromic deafness 1A X-linked mixed deafness with perilymphatic gusher hidradenitis suppurativa autosomal dominant nonsyndromic deafness 3A porokeratotic eccrine ostial and dermal duct nevus skin cancer KID syndrome skin neoplasm autosomal dominant keratitis-ichthyosis-deafness syndrome cellulitis Deafness pancreatic intraductal papillary-mucinous neoplasm ductal breast carcinoma in situ head and neck cancer intellectual disability pustulosis palmaris et plantaris atopic eczema squamous cell carcinoma autosomal recessive nonsyndromic deafness 12 lung adenocarcinoma vestibular disease malignant glioma breast carcinoma cystic fibrosis ovarian cancer pancreatic ductal adenocarcinoma Pendred syndrome erythrokeratoderma invasive ductal breast carcinoma tuberculosis mal de Meleda malignant pancreatic neoplasm non-small cell lung carcinoma deafness, autosomal recessive pancreatic intraductal papillary-mucinous carcinoma facioscapulohumeral muscular dystrophy primitive neuroectodermal tumor spina bifida large cell medulloblastoma Usher syndrome autosomal recessive nonsyndromic deafness 1B malignant ependymoma breast cancer medulloblastoma

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, D, E A, B, C, D, E, F	OPM	Transmembrane	Alpha-helical polytopic	Tetraspan junctional complexes	Connexin

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C, D, E A, B, C, D, E, F	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B, C, D, E A, B, C, D, E, F	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B, C, D, E A, B, C, D, E, F	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	Channels: Gap Junctions and Related Channels	Connexin 26 (Cx26; GJB2) gap junction

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.