2VSU

A ternary complex of Hydroxycinnamoyl-CoA Hydratase-Lyase (HCHL) with acetyl-Coenzyme A and vanillin gives insights into substrate specificity and mechanism.

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2vsua_	Alpha and beta proteins (a/b)	ClpP/crotonase	ClpP/crotonase	automated matches	automated matches	(Pseudomonas fluorescens ) [TaxId: 294 ],	SCOPe (2.08)
B	d2vsub_	Alpha and beta proteins (a/b)	ClpP/crotonase	ClpP/crotonase	automated matches	automated matches	(Pseudomonas fluorescens ) [TaxId: 294 ],	SCOPe (2.08)
D	d2vsud_	Alpha and beta proteins (a/b)	ClpP/crotonase	ClpP/crotonase	automated matches	automated matches	(Pseudomonas fluorescens ) [TaxId: 294 ],	SCOPe (2.08)
C	d2vsuc_	Alpha and beta proteins (a/b)	ClpP/crotonase	ClpP/crotonase	automated matches	automated matches	(Pseudomonas fluorescens ) [TaxId: 294 ],	SCOPe (2.08)
E	d2vsue_	Alpha and beta proteins (a/b)	ClpP/crotonase	ClpP/crotonase	automated matches	automated matches	(Pseudomonas fluorescens ) [TaxId: 294 ],	SCOPe (2.08)
F	d2vsuf_	Alpha and beta proteins (a/b)	ClpP/crotonase	ClpP/crotonase	automated matches	automated matches	(Pseudomonas fluorescens ) [TaxId: 294 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	ClpP/crotonase	8067193	3000033	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	ClpP/crotonase	8067193	3000033	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	ClpP/crotonase	8067193	3000033	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	ClpP/crotonase	8067193	3000033	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	ClpP/crotonase	8067193	3000033	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	ClpP/crotonase	8067193	3000033	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00378	e2vsuA1	A: a/b three-layered sandwiches	X: ClpP/crotonase (From Topology)	H: ClpP/crotonase (From Topology)	T: ClpP/crotonase	F: PF00378	ECOD (1.6)
B	PF00378	e2vsuB1	A: a/b three-layered sandwiches	X: ClpP/crotonase (From Topology)	H: ClpP/crotonase (From Topology)	T: ClpP/crotonase	F: PF00378	ECOD (1.6)
D	PF00378	e2vsuD1	A: a/b three-layered sandwiches	X: ClpP/crotonase (From Topology)	H: ClpP/crotonase (From Topology)	T: ClpP/crotonase	F: PF00378	ECOD (1.6)
C	PF00378	e2vsuC1	A: a/b three-layered sandwiches	X: ClpP/crotonase (From Topology)	H: ClpP/crotonase (From Topology)	T: ClpP/crotonase	F: PF00378	ECOD (1.6)
E	PF00378	e2vsuE1	A: a/b three-layered sandwiches	X: ClpP/crotonase (From Topology)	H: ClpP/crotonase (From Topology)	T: ClpP/crotonase	F: PF00378	ECOD (1.6)
F	PF00378	e2vsuF1	A: a/b three-layered sandwiches	X: ClpP/crotonase (From Topology)	H: ClpP/crotonase (From Topology)	T: ClpP/crotonase	F: PF00378	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.90.226.10	Alpha Beta	Alpha-Beta Complex	2-enoyl-CoA Hydratase	Chain A, domain 1	CATH (4.3.0)
A	6.10.250.2850	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
B	3.90.226.10	Alpha Beta	Alpha-Beta Complex	2-enoyl-CoA Hydratase	Chain A, domain 1	CATH (4.3.0)
B	6.10.250.2850	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
D	3.90.226.10	Alpha Beta	Alpha-Beta Complex	2-enoyl-CoA Hydratase	Chain A, domain 1	CATH (4.3.0)
D	6.10.250.2850	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
C	3.90.226.10	Alpha Beta	Alpha-Beta Complex	2-enoyl-CoA Hydratase	Chain A, domain 1	CATH (4.3.0)
C	6.10.250.2850	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
E	3.90.226.10	Alpha Beta	Alpha-Beta Complex	2-enoyl-CoA Hydratase	Chain A, domain 1	CATH (4.3.0)
E	6.10.250.2850	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
F	3.90.226.10	Alpha Beta	Alpha-Beta Complex	2-enoyl-CoA Hydratase	Chain A, domain 1	CATH (4.3.0)
F	6.10.250.2850	Special	Helix non-globular	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, D	PF00378	Enoyl-CoA hydratase/isomerase (ECH_1)	Enoyl-CoA hydratase/isomerase	This family contains a diverse set of enzymes including: enoyl-CoA hydratase, napthoate synthase, carnitate racemase, 3-hydroxybutyryl-CoA dehydratase and dodecanoyl-CoA delta-isomerase.	Domain
C	PF00378	Enoyl-CoA hydratase/isomerase (ECH_1)	Enoyl-CoA hydratase/isomerase	This family contains a diverse set of enzymes including: enoyl-CoA hydratase, napthoate synthase, carnitate racemase, 3-hydroxybutyryl-CoA dehydratase and dodecanoyl-CoA delta-isomerase.	Domain
E	PF00378	Enoyl-CoA hydratase/isomerase (ECH_1)	Enoyl-CoA hydratase/isomerase	This family contains a diverse set of enzymes including: enoyl-CoA hydratase, napthoate synthase, carnitate racemase, 3-hydroxybutyryl-CoA dehydratase and dodecanoyl-CoA delta-isomerase.	Domain
F	PF00378	Enoyl-CoA hydratase/isomerase (ECH_1)	Enoyl-CoA hydratase/isomerase	This family contains a diverse set of enzymes including: enoyl-CoA hydratase, napthoate synthase, carnitate racemase, 3-hydroxybutyryl-CoA dehydratase and dodecanoyl-CoA delta-isomerase.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, D	P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE	feruloyl-CoA hydratase/lyase activity lyase activity catalytic activity carbon-carbon lyase activity aldehyde-lyase activity	isoprenoid catabolic process lipid catabolic process catabolic process cellular metabolic process primary metabolic process cellular lipid catabolic process cellular catabolic process organic substance metabolic process cellular lipid metabolic process metabolic process cellular process organic substance catabolic process lipid metabolic process isoprenoid metabolic process	-
C	P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE	feruloyl-CoA hydratase/lyase activity lyase activity catalytic activity carbon-carbon lyase activity aldehyde-lyase activity	isoprenoid catabolic process lipid catabolic process catabolic process cellular metabolic process primary metabolic process cellular lipid catabolic process cellular catabolic process organic substance metabolic process cellular lipid metabolic process metabolic process cellular process organic substance catabolic process lipid metabolic process isoprenoid metabolic process	-
E	P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE	feruloyl-CoA hydratase/lyase activity lyase activity catalytic activity carbon-carbon lyase activity aldehyde-lyase activity	isoprenoid catabolic process lipid catabolic process catabolic process cellular metabolic process primary metabolic process cellular lipid catabolic process cellular catabolic process organic substance metabolic process cellular lipid metabolic process metabolic process cellular process organic substance catabolic process lipid metabolic process isoprenoid metabolic process	-
F	P-HYDROXYCINNAMOYL COA HYDRATASE/LYASE	feruloyl-CoA hydratase/lyase activity lyase activity catalytic activity carbon-carbon lyase activity aldehyde-lyase activity	isoprenoid catabolic process lipid catabolic process catabolic process cellular metabolic process primary metabolic process cellular lipid catabolic process cellular catabolic process organic substance metabolic process cellular lipid metabolic process metabolic process cellular process organic substance catabolic process lipid metabolic process isoprenoid metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, D	IPR001753	Enoyl-CoA hydratase/isomerase	Family
A, B, D	IPR029045	ClpP/crotonase-like domain superfamily	Homologous Superfamily
A, B, D	IPR018376	Enoyl-CoA hydratase/isomerase, conserved site	Conserved Site
C	IPR001753	Enoyl-CoA hydratase/isomerase	Family
C	IPR029045	ClpP/crotonase-like domain superfamily	Homologous Superfamily
C	IPR018376	Enoyl-CoA hydratase/isomerase, conserved site	Conserved Site
E	IPR001753	Enoyl-CoA hydratase/isomerase	Family
E	IPR029045	ClpP/crotonase-like domain superfamily	Homologous Superfamily
E	IPR018376	Enoyl-CoA hydratase/isomerase, conserved site	Conserved Site
F	IPR001753	Enoyl-CoA hydratase/isomerase	Family
F	IPR029045	ClpP/crotonase-like domain superfamily	Homologous Superfamily
F	IPR018376	Enoyl-CoA hydratase/isomerase, conserved site	Conserved Site

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.