This family contains a diverse set of proteins with a thioredoxin-like structure Pfam:PF00085. This family also includes 2-hydroxychromene-2-carboxylate (HCCA) isomerase enzymes catalyse one step in prokaryotic polyaromatic hydrocarbon (PAH) cataboli ...
This family contains a diverse set of proteins with a thioredoxin-like structure Pfam:PF00085. This family also includes 2-hydroxychromene-2-carboxylate (HCCA) isomerase enzymes catalyse one step in prokaryotic polyaromatic hydrocarbon (PAH) catabolic pathways [2,3,4]. This family also contains members with functions other than HCCA isomerisation, such as Kappa family GSTs (e.g. Swiss:P24473), whose similarity to HCCA isomerases was not previously recognised. The sequence Swiss:O07298 has been annotated as a dioxygenase but is almost certainly an HCCA isomerase enzyme. Similarly, the sequence Swiss:Q9ZI67 has been annotated as a dehydrogenase, but is most probably also an HCCA isomerase enzyme. In addition, the Rhizobium leguminosarum Swiss:Q52782 protein has been annotated as a putative glycerol-3-phosphate transfer protein, but is also most likely to be an HCCA isomerase enzyme (see [5]).
Catalyzes the reversible glutathione-dependent isomerization of 2-hydroxychromene-2-carboxylate (HCCA) to trans-O-hydroxybenzylidenepyruvate (THBPA). HCCA isomerases are present in most aerobic organisms including microorganisms, plants, and animals. The group kappa GSH transferases (k-GSTs) are a close relative of the largest group of proteins in nature; the GST group. k-GSTs are dimers with a main catalytic domain, which is the N-terminal domain containing the thioredoxin domain, and the C-terminal domain which is mostly alfa-helical and unlike its GST relatives, inserted into the N-terminal domain. HCCA is a part of the naphthalene catabolic pathway of Pseudomonas putida.
Defined by 5 residues: SER:A-11VAL:A-168TRP:A-179ASN:A-181ASP:A-182
