2ADM

ADENINE-N6-DNA-METHYLTRANSFERASE TAQI

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Structure Motif: Primary M-CSA Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2adma1	Alpha and beta proteins (a/b)	S-adenosyl-L-methionine-dependent methyltransferases	S-adenosyl-L-methionine-dependent methyltransferases	DNA methylase TaqI, N-terminal domain	DNA methylase TaqI, N-terminal domain	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
A	d2adma2	Alpha and beta proteins (a+b)	DNA methylase specificity domain	DNA methylase specificity domain	TaqI C-terminal domain-like	DNA methylase TaqI, C-terminal domain	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
B	d2admb1	Alpha and beta proteins (a/b)	S-adenosyl-L-methionine-dependent methyltransferases	S-adenosyl-L-methionine-dependent methyltransferases	DNA methylase TaqI, N-terminal domain	DNA methylase TaqI, N-terminal domain	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)
B	d2admb2	Alpha and beta proteins (a+b)	DNA methylase specificity domain	DNA methylase specificity domain	TaqI C-terminal domain-like	DNA methylase TaqI, C-terminal domain	(Thermus aquaticus ) [TaxId: 271 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8034512	3000118	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	DNA methylase specificity domain	8034513	3001742	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	S-adenosyl-L-methionine-dependent methyltransferases	8034512	3000118	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	DNA methylase specificity domain	8034513	3001742	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	TaqI_C	e2admA1	A: a+b complex topology	X: DNA methylase specificity domain (From Topology)	H: DNA methylase specificity domain (From Topology)	T: DNA methylase specificity domain	F: TaqI_C	ECOD (1.6)
A	N6_Mtase	e2admA2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: N6_Mtase	ECOD (1.6)
B	TaqI_C	e2admB1	A: a+b complex topology	X: DNA methylase specificity domain (From Topology)	H: DNA methylase specificity domain (From Topology)	T: DNA methylase specificity domain	F: TaqI_C	ECOD (1.6)
B	N6_Mtase	e2admB2	A: a/b three-layered sandwiches	X: Rossmann-like	H: Rossmann-related	T: S-adenosyl-L-methionine-dependent methyltransferases	F: N6_Mtase	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
A	3.90.220.10	Alpha Beta	Alpha-Beta Complex	Adenine-n6-DNA-methyltransferase TaqI	Chain A, domain 2	CATH (4.3.0)
B	3.40.50.150	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Vaccinia Virus protein VP39	CATH (4.3.0)
B	3.90.220.10	Alpha Beta	Alpha-Beta Complex	Adenine-n6-DNA-methyltransferase TaqI	Chain A, domain 2	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF12950	TaqI-like C-terminal specificity domain (TaqI_C)	TaqI-like C-terminal specificity domain	This domain is found at the C-terminus of the TaqI protein and is involved in DNA-binding and substrate recognition.	Domain
A, B	PF07669	Eco57I restriction-modification methylase (Eco57I)	Eco57I restriction-modification methylase	Homologues of the Escherichia coli Eco57I restriction-modification methylase are found in several phylogenetically diverse bacteria. The structure of TaqI has been solved [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	ADENINE-N6-DNA-METHYLTRANSFERASE TAQI	DNA binding binding organic cyclic compound binding nucleic acid binding DNA-methyltransferase activity catalytic activity, acting on DNA site-specific DNA-methyltransferase (adenine-specific) activity transferase activity, transferring one-carbon groups catalytic activity, acting on a nucleic acid transferase activity methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity catalytic activity	biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus biological process involved in interspecies interaction between organisms response to stimulus DNA restriction-modification system clearance of foreign intracellular nucleic acids response to biotic stimulus nucleobase-containing compound metabolic process DNA modification organic substance metabolic process response to stress response to external biotic stimulus response to other organism defense response defense response to symbiont response to external stimulus primary metabolic process clearance of foreign intracellular DNA DNA metabolic process nucleic acid metabolic process organic cyclic compound metabolic process defense response to other organism macromolecule modification metabolic process macromolecule metabolic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR002052	DNA methylase, N-6 adenine-specific, conserved site	Conserved Site
A, B	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase superfamily	Homologous Superfamily
A, B	IPR025931	TaqI-like C-terminal specificity domain	Domain
A, B	IPR021188	N6 adenine-specific DNA methyltransferase, TaqI class	Family
A, B	IPR023135	N6 adenine-specific DNA methyltransferase, TaqI class, C-terminal	Homologous Superfamily
A, B	IPR011639	Type II methyltransferase M.TaqI-like domain	Domain

Structure Motif Annotation: Mechanism and Catalytic Site Atlas M-CSA Database Homepage

Chains	Enzyme Name	Description	Catalytic Residues
A	site-specific DNA-methyltransferase (adenine-specific) M-CSA #46	N-6 adenine-specific DNA-methyltransferases catalyse the transfer of a methyl group from S-adenosyl-L-methionine to either the exocyclic N-6 of an adenine or to N-4 of a cytosine contained in a specific DNA recognition sequence. Methylation protects the DNA from restriction from host endonucleases.	Defined by 4 residues: ASN:A-105PRO:A-106TYR:A-108PHE:A-196 \| Explore in 3D: M-CSA Motif Definition Search M-CSA Motif EC: 2.1.1.72 (PDB Primary Data) Search M-CSA Motif + EC 2.1.1.72

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.