1XDT

COMPLEX OF DIPHTHERIA TOXIN AND HEPARIN-BINDING EPIDERMAL GROWTH FACTOR

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A [auth T]	d1xdtt1	All beta proteins	Common fold of diphtheria toxin/transcription factors/cytochrome f	Diphtheria toxin, C-terminal domain	Diphtheria toxin, C-terminal domain	Diphtheria toxin, C-terminal domain	(Corynebacterium diphtheriae ) [TaxId: 1717 ],	SCOPe (2.08)
A [auth T]	d1xdtt2	Alpha and beta proteins (a+b)	ADP-ribosylation	ADP-ribosylation	ADP-ribosylating toxins	Diphtheria toxin, N-terminal domain	(Corynebacterium diphtheriae ) [TaxId: 1717 ],	SCOPe (2.08)
A [auth T]	d1xdtt3	Membrane and cell surface proteins and peptides	Toxins' membrane translocation domains	Diphtheria toxin, middle domain	Diphtheria toxin, middle domain	Diphtheria toxin, middle domain	(Corynebacterium diphtheriae ) [TaxId: 1717 ],	SCOPe (2.08)
B [auth R]	d1xdtr_	Small proteins	Knottins (small inhibitors, toxins, lectins)	EGF/Laminin	EGF-type module	Heparin-binding epidermal growth factor, HBEGF	human (Homo sapiens ) [TaxId: 9606 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A [auth T]	SCOP2B Superfamily	Diphtheria toxin C-terminal domain	8040181	3000694	SCOP2B (2022-06-29)
A [auth T]	SCOP2B Superfamily	Diphtheria toxin middle domain	8037645	3001959	SCOP2B (2022-06-29)
A [auth T]	SCOP2B Superfamily	ADP-ribosylation	8036997	3001208	SCOP2B (2022-06-29)
B [auth R]	SCOP2 Family	EGF-type module	8025957	4003198	SCOP2 (2022-06-29)
B [auth R]	SCOP2 Superfamily	EGF/Laminin-like	8038336	3000412	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A [auth T]	PF01324	e1xdtT1	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Immunoglobulin-related	T: Common fold of diphtheria toxin/transcription factors/cytochrome f	F: PF01324	ECOD (1.6)
A [auth T]	PF02764	e1xdtT2	A: alpha arrays	X: Toxins' membrane translocation domains	H: Diphtheria toxin, middle domain (From Topology)	T: Diphtheria toxin, middle domain	F: PF02764	ECOD (1.6)
A [auth T]	PF02763	e1xdtT3	A: a+b complex topology	X: ADP-ribosylation (From Topology)	H: ADP-ribosylation (From Topology)	T: ADP-ribosylation	F: PF02763	ECOD (1.6)
B [auth R]	PF00008	e1xdtR1	A: few secondary structure elements	X: EGF-like	H: EGF-related	T: EGF/Laminin	F: PF00008	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A [auth T]	3.90.175.10	Alpha Beta	Alpha-Beta Complex	Diphtheria Toxin	domain 1	CATH (4.3.0)
A [auth T]	1.10.490.40	Mainly Alpha	Orthogonal Bundle	Globin-like	Diphtheria toxin, translocation domain	CATH (4.3.0)
A [auth T]	2.60.40.700	Mainly Beta	Sandwich	Immunoglobulin-like	Diphtheria toxin, receptor-binding domain	CATH (4.3.0)
B [auth R]	2.10.25.10	Mainly Beta	Ribbon	Laminin	Laminin	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A [auth T]	PF02764	Diphtheria toxin, T domain (Diphtheria_T)	Diphtheria toxin, T domain	Central domain of diphtheria toxin is the translocation (T) domain. pH induced conformational change in this domain triggers insertion into the endosomal membrane and facilitates the transfer of the catalytic domain into the cytoplasm.	Domain
A [auth T]	PF02763	Diphtheria toxin, C domain (Diphtheria_C)	Diphtheria toxin, C domain	N-terminal catalytic (C) domain - blocks protein synthesis by transfer of ADP-ribose from NAD to a diphthamide residue of EF-2.	Domain
A [auth T]	PF01324	Diphtheria toxin, R domain (Diphtheria_R)	Diphtheria toxin, R domain	C-terminal receptor binding (R) domain - binds to cell surface receptor, permitting the toxin to enter the cell by receptor mediated endocytosis.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A [auth T]	DIPHTHERIA TOXIN	macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity transporter activity transmembrane transporter activity pentosyltransferase activity glycosyltransferase activity NAD+-diphthamide ADP-ribosyltransferase activity transferase activity catalytic activity toxin activity nucleotidyltransferase activity transferase activity, transferring phosphorus-containing groups identical protein binding macromolecule transmembrane transporter activity protein transporter activity protein transmembrane transporter activity transporter activity transmembrane transporter activity pentosyltransferase activity glycosyltransferase activity NAD+-diphthamide ADP-ribosyltransferase activity transferase activity catalytic activity toxin activity nucleotidyltransferase activity transferase activity, transferring phosphorus-containing groups identical protein binding protein binding binding	-	extracellular region cellular anatomical entity cell periphery plasma membrane membrane extracellular space
B [auth R]	HEPARIN-BINDING EPIDERMAL GROWTH FACTOR	protein binding binding molecular function activator activity signaling receptor binding molecular function regulator activity signaling receptor regulator activity receptor ligand activity signaling receptor activator activity growth factor activity protein tyrosine kinase activator activity enzyme regulator activity transmembrane receptor protein tyrosine kinase activator activity protein kinase regulator activity kinase activator activity protein binding binding molecular function activator activity signaling receptor binding molecular function regulator activity signaling receptor regulator activity receptor ligand activity signaling receptor activator activity growth factor activity protein tyrosine kinase activator activity enzyme regulator activity transmembrane receptor protein tyrosine kinase activator activity protein kinase regulator activity kinase activator activity enzyme activator activity protein kinase activator activity kinase regulator activity epidermal growth factor receptor binding growth factor receptor binding heparin binding glycosaminoglycan binding carbohydrate derivative binding sulfur compound binding	regulation of biological process regulation of growth regulation of cellular process regulation of cellular component organization positive regulation of biological process biological regulation growth positive regulation of cell growth regulation of cell growth cell growth cellular component organization positive regulation of growth cellular process cellular component organization or biogenesis regulation of biological process regulation of growth regulation of cellular process regulation of cellular component organization positive regulation of biological process biological regulation growth positive regulation of cell growth regulation of cell growth cell growth cellular component organization positive regulation of growth cellular process cellular component organization or biogenesis positive regulation of cellular process heart process multicellular organismal process system process regulation of heart contraction regulation of system process circulatory system process regulation of blood circulation regulation of multicellular organismal process heart contraction blood circulation cellular response to stimulus ERBB2 signaling pathway ERBB4 signaling pathway cell surface receptor signaling pathway response to stimulus signaling cell surface receptor protein tyrosine kinase signaling pathway cell communication ERBB signaling pathway signal transduction enzyme-linked receptor protein signaling pathway ERBB2-ERBB4 signaling pathway positive regulation of response to wounding wound healing regulation of response to wounding positive regulation of response to stimulus positive regulation of wound healing regulation of response to stimulus response to stress regulation of response to stress regulation of wound healing response to wounding developmental process muscle structure development animal organ development anatomical structure development muscle organ development positive regulation of cell communication regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction positive regulation of signal transduction regulation of signal transduction intracellular signal transduction regulation of intracellular signal transduction intracellular signaling cassette regulation of signaling positive regulation of intracellular signal transduction positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction regulation of cell communication phosphatidylinositol 3-kinase/protein kinase B signal transduction positive regulation of signaling cell population proliferation positive regulation of cell population proliferation regulation of cell population proliferation cellular biosynthetic process organonitrogen compound biosynthetic process negative regulation of biological process carbohydrate derivative metabolic process negative regulation of cellular metabolic process regulation of primary metabolic process organic substance metabolic process organic substance biosynthetic process negative regulation of cellular process protein metabolic process regulation of glycoprotein biosynthetic process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process carbohydrate derivative biosynthetic process regulation of biosynthetic process negative regulation of glycoprotein biosynthetic process negative regulation of glycoprotein metabolic process organonitrogen compound metabolic process negative regulation of elastin biosynthetic process cellular metabolic process regulation of protein metabolic process negative regulation of metabolic process glycoprotein biosynthetic process primary metabolic process regulation of cellular metabolic process regulation of macromolecule metabolic process glycoprotein metabolic process macromolecule biosynthetic process elastin metabolic process negative regulation of cellular biosynthetic process regulation of glycoprotein metabolic process regulation of cellular biosynthetic process biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process metabolic process regulation of macromolecule biosynthetic process macromolecule metabolic process regulation of elastin biosynthetic process elastin biosynthetic process regulation of metabolic process epidermal growth factor receptor signaling pathway cell chemotaxis chemotaxis response to external stimulus cellular response to chemical stimulus taxis response to chemical cell migration cell motility locomotion epithelium development morphogenesis of an epithelium epiboly involved in wound healing tissue development morphogenesis of an epithelial sheet wound healing, spreading of epidermal cells tissue morphogenesis anatomical structure morphogenesis epiboly wound healing, spreading of cells positive regulation of locomotion regulation of cell motility regulation of keratinocyte migration positive regulation of cell migration positive regulation of epithelial cell migration tissue migration ameboidal-type cell migration positive regulation of cell motility regulation of cell migration epithelial cell migration regulation of epithelial cell migration epithelium migration regulation of locomotion positive regulation of keratinocyte migration keratinocyte migration regulation of smooth muscle cell proliferation smooth muscle cell proliferation positive regulation of smooth muscle cell proliferation muscle cell proliferation ERBB2-EGFR signaling pathway regulation of protein modification process positive regulation of phosphorylation regulation of protein phosphorylation positive regulation of protein metabolic process positive regulation of cellular metabolic process peptidyl-tyrosine modification positive regulation of phosphate metabolic process positive regulation of metabolic process positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein modification process regulation of phosphate metabolic process peptidyl-amino acid modification phosphate-containing compound metabolic process positive regulation of macromolecule metabolic process protein modification process phosphorus metabolic process positive regulation of protein phosphorylation protein phosphorylation positive regulation of phosphorus metabolic process phosphorylation regulation of peptidyl-tyrosine phosphorylation macromolecule modification regulation of phosphorylation regulation of phosphorus metabolic process peptidyl-tyrosine phosphorylation	membrane-bounded organelle intracellular anatomical structure intracellular vesicle intracellular organelle bounding membrane of organelle cytoplasmic vesicle vesicle membrane clathrin-coated vesicle endocytic vesicle membrane coated vesicle vesicle endocytic vesicle clathrin-coated vesicle membrane clathrin-coated endocytic vesicle membrane membrane-bounded organelle intracellular anatomical structure intracellular vesicle intracellular organelle bounding membrane of organelle cytoplasmic vesicle vesicle membrane clathrin-coated vesicle endocytic vesicle membrane coated vesicle vesicle endocytic vesicle clathrin-coated vesicle membrane clathrin-coated endocytic vesicle membrane membrane cellular anatomical entity intracellular membrane-bounded organelle clathrin-coated endocytic vesicle cytoplasmic vesicle membrane coated vesicle membrane organelle cytoplasm organelle membrane cell surface cell periphery plasma membrane extracellular region extracellular space

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A [auth T]	IPR036799	Diphtheria toxin, receptor-binding domain superfamily	Homologous Superfamily
A [auth T]	IPR000512	Diphtheria toxin (NAD+-dipthamide ADP-ribosyltransferase)	Family
A [auth T]	IPR022404	Diphtheria toxin, receptor-binding domain	Domain
A [auth T]	IPR036801	Diphtheria toxin, translocation domain superfamily	Homologous Superfamily
A [auth T]	IPR022406	Diphtheria toxin, catalytic domain	Domain
B [auth R]	IPR000742	EGF-like domain	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
B [auth R]	Pharos: Q99075	perinatal necrotizing enterocolitis epilepsy with generalized tonic-clonic seizures gastric cancer juvenile idiopathic arthritis chronic kidney disease endometriosis Hypertrophy, Left Ventricular lung carcinoma acute kidney failure MALT lymphoma breast cancer hepatocellular carcinoma interstitial cystitis lung adenocarcinoma malignant mesothelioma perinatal necrotizing enterocolitis epilepsy with generalized tonic-clonic seizures gastric cancer juvenile idiopathic arthritis chronic kidney disease endometriosis Hypertrophy, Left Ventricular lung carcinoma acute kidney failure MALT lymphoma breast cancer hepatocellular carcinoma interstitial cystitis lung adenocarcinoma malignant mesothelioma necrotizing enterocolitis pituitary cancer cystic fibrosis sinusitis gastric carcinoma hereditary diffuse gastric adenocarcinoma ischemia reperfusion injury large cell medulloblastoma lung cancer non-small cell lung carcinoma psoriasis diabetes mellitus ulcerative colitis gastric neoplasm

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.