1FU5

NMR STRUCTURE OF THE N-SH2 DOMAIN OF THE P85 SUBUNIT OF PI3-KINASE COMPLEXED TO A DOUBLY PHOSPHORYLATED PEPTIDE DERIVED FROM POLYOMAVIRUS MIDDLE T ANTIGEN

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Protein Modification Annotation

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1fu5a_	Alpha and beta proteins (a+b)	SH2-like	SH2 domain	SH2 domain	Phosphatidylinositol 3-kinase, p85-alpha subunit	Norway rat (Rattus norvegicus ) [TaxId: 10116 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	SH2 domain	8035668	3000197	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PF00017	e1fu5A1	A: a+b two layers	X: SH2 (From Topology)	H: SH2 (From Topology)	T: SH2	F: PF00017	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.505.10	Alpha Beta	2-Layer Sandwich	SHC Adaptor Protein	SH2 domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00017	SH2 domain (SH2)	SH2 domain	-	Domain
A	PF16454	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain (PI3K_P85_iSH2)	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain	This domain is found between the two SH2 domains in phosphatidylinositol 3-kinase regulatory subunit P85. It forms a complex with the adaptor-binding domain of phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY ALPHA SUBUNIT	amide binding protein binding binding peptide hormone binding hormone binding insulin binding signaling receptor binding platelet-derived growth factor receptor binding growth factor receptor binding protein phosphatase binding phosphatase binding enzyme binding enzyme regulator activity molecular function regulator activity amide binding protein binding binding peptide hormone binding hormone binding insulin binding signaling receptor binding platelet-derived growth factor receptor binding growth factor receptor binding protein phosphatase binding phosphatase binding enzyme binding enzyme regulator activity molecular function regulator activity phosphatidylinositol 3-kinase regulator activity kinase regulator activity calmodulin binding ubiquitin protein ligase binding ubiquitin-like protein ligase binding insulin-like growth factor receptor binding protein domain specific binding kinase binding protein kinase binding ErbB-3 class receptor binding transcription factor binding RNA polymerase II-specific DNA-binding transcription factor binding nuclear receptor binding DNA-binding transcription factor binding nuclear estrogen receptor binding insulin receptor substrate binding phosphoprotein binding phosphatidylinositol kinase activity kinase activity lipid kinase activity transferase activity catalytic activity 1-phosphatidylinositol-3-kinase activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups insulin receptor binding kinase activator activity enzyme activator activity molecular function activator activity phosphotyrosine residue binding protein phosphorylated amino acid binding neurotrophin TRK receptor binding neurotrophin TRKA receptor binding cytokine receptor binding neurotrophin receptor binding protein tyrosine kinase binding receptor tyrosine kinase binding ATPase binding 1-phosphatidylinositol-3-kinase regulator activity protein heterodimerization activity protein dimerization activity phosphatidylinositol 3-kinase binding phosphatidylinositol 3-kinase regulatory subunit binding	response to stimulus response to chemical response to xenobiotic stimulus developmental process regulation of biological process regulation of cellular process regulation of developmental process cellular developmental process biological regulation positive regulation of biological process regulation of cell differentiation positive regulation of myoblast differentiation muscle structure development positive regulation of developmental process response to stimulus response to chemical response to xenobiotic stimulus developmental process regulation of biological process regulation of cellular process regulation of developmental process cellular developmental process biological regulation positive regulation of biological process regulation of cell differentiation positive regulation of myoblast differentiation muscle structure development positive regulation of developmental process positive regulation of cell differentiation cell differentiation anatomical structure development regulation of myoblast differentiation cellular process myoblast differentiation positive regulation of cellular process regulation of protein stability regulation of biological quality protein stabilization response to organic substance response to ketone response to lipid response to oxygen-containing compound response to testosterone response to organic cyclic compound biological process involved in interspecies interaction between organisms response to other organism response to yeast response to external stimulus response to fungus response to external biotic stimulus response to biotic stimulus response to hexose response to monosaccharide response to carbohydrate response to fructose response to iron(II) ion response to metal ion response to iron ion response to inorganic substance cellular response to stimulus cell surface receptor signaling pathway signaling insulin-like growth factor receptor signaling pathway cell surface receptor protein tyrosine kinase signaling pathway cell communication signal transduction enzyme-linked receptor protein signaling pathway regulation of cell adhesion negative regulation of biological process negative regulation of cell adhesion cell adhesion cell-cell adhesion negative regulation of cell-cell adhesion negative regulation of cellular process regulation of cell-cell adhesion response to endogenous stimulus response to glucocorticoid response to corticosteroid response to hormone response to steroid hormone response to dexamethasone cell population proliferation regulation of smooth muscle cell proliferation positive regulation of cell population proliferation smooth muscle cell proliferation positive regulation of smooth muscle cell proliferation regulation of cell population proliferation muscle cell proliferation protein localization regulation of localization cellular localization protein localization to cell periphery regulation of protein localization protein localization to plasma membrane localization within membrane regulation of protein localization to cell periphery protein localization to membrane localization regulation of cellular localization cellular macromolecule localization macromolecule localization regulation of protein localization to plasma membrane regulation of protein localization to membrane response to organonitrogen compound response to organophosphorus response to nitrogen compound response to purine-containing compound response to cAMP cellular response to stress response to stress response to endoplasmic reticulum stress positive regulation of nervous system development multicellular organismal process positive regulation of cellular component organization regulation of cellular component organization regulation of nervous system development cellular component organization cellular component biogenesis multicellular organism development cellular component assembly cell junction assembly regulation of multicellular organismal process regulation of synapse structure or activity synapse organization cellular component organization or biogenesis regulation of synapse assembly system development regulation of multicellular organismal development positive regulation of cellular component biogenesis nervous system development regulation of cellular component biogenesis regulation of cell junction assembly positive regulation of cell junction assembly regulation of synapse organization cell junction organization positive regulation of multicellular organismal process synapse assembly positive regulation of synapse assembly negative regulation of programmed cell death regulation of apoptotic process regulation of programmed cell death cell death negative regulation of apoptotic process apoptotic process programmed cell death regulation of cytoskeleton organization regulation of actin filament-based process regulation of actin filament bundle assembly actin filament bundle organization actin filament-based process stress fiber assembly negative regulation of supramolecular fiber organization regulation of actin filament organization negative regulation of stress fiber assembly actin cytoskeleton organization actomyosin structure organization negative regulation of cellular component organization negative regulation of cytoskeleton organization actin filament organization negative regulation of actin filament bundle assembly regulation of organelle organization regulation of stress fiber assembly organelle organization negative regulation of organelle organization contractile actin filament bundle assembly regulation of actomyosin structure organization actin filament bundle assembly regulation of actin cytoskeleton organization regulation of supramolecular fiber organization supramolecular fiber organization cytoskeleton organization negative regulation of proteolysis organonitrogen compound metabolic process regulation of protein metabolic process negative regulation of metabolic process primary metabolic process regulation of macromolecule metabolic process regulation of primary metabolic process organic substance metabolic process regulation of proteolysis proteolysis metabolic process protein metabolic process macromolecule metabolic process regulation of metabolic process negative regulation of protein metabolic process negative regulation of macromolecule metabolic process cellular biosynthetic process RNA metabolic process positive regulation of cellular metabolic process positive regulation of macromolecule biosynthetic process nucleobase-containing compound metabolic process positive regulation of metabolic process regulation of RNA metabolic process positive regulation of biosynthetic process organic substance biosynthetic process positive regulation of macromolecule metabolic process RNA processing regulation of nucleobase-containing compound metabolic process regulation of biosynthetic process RNA splicing positive regulation of nucleobase-containing compound metabolic process cellular metabolic process regulation of cellular metabolic process gene expression regulation of RNA splicing macromolecule biosynthetic process nucleic acid metabolic process positive regulation of RNA metabolic process regulation of cellular biosynthetic process organic cyclic compound metabolic process regulation of gene expression biosynthetic process positive regulation of gene expression regulation of macromolecule biosynthetic process positive regulation of RNA splicing positive regulation of cellular biosynthetic process positive regulation of locomotion regulation of cell motility regulation of leukocyte migration positive regulation of leukocyte migration positive regulation of cell migration leukocyte migration immune system process regulation of locomotion positive regulation of cell motility regulation of cell migration cell migration regulation of immune system process cell motility positive regulation of immune system process locomotion cellular response to nitrogen compound response to peptide hormone insulin receptor signaling pathway cellular response to endogenous stimulus cellular response to organic substance cellular response to hormone stimulus cellular response to peptide hormone stimulus cellular response to oxygen-containing compound cellular response to chemical stimulus cellular response to organonitrogen compound response to insulin cellular response to insulin stimulus response to growth factor regulation of cell-substrate adhesion cell-substrate adhesion negative regulation of cell-matrix adhesion cell-matrix adhesion regulation of cell-matrix adhesion negative regulation of cell-substrate adhesion myeloid leukocyte migration myeloid leukocyte differentiation hemopoiesis cell development myeloid cell differentiation osteoclast differentiation leukocyte differentiation filopodium assembly cell projection organization regulation of filopodium assembly plasma membrane bounded cell projection assembly positive regulation of cell projection organization positive regulation of filopodium assembly plasma membrane bounded cell projection organization regulation of cell projection organization regulation of cell projection assembly regulation of plasma membrane bounded cell projection assembly cell projection assembly regulation of plasma membrane bounded cell projection organization positive regulation of plasma membrane bounded cell projection assembly response to fatty acid positive regulation of response to biotic stimulus immune response-regulating cell surface receptor signaling pathway pattern recognition receptor signaling pathway regulation of signal transduction immune response-activating signaling pathway positive regulation of immune response cell surface toll-like receptor signaling pathway innate immune response-activating signaling pathway immune response immune response-activating cell surface receptor signaling pathway regulation of innate immune response regulation of toll-like receptor 4 signaling pathway regulation of response to stimulus defense response to other organism regulation of pattern recognition receptor signaling pathway positive regulation of defense response activation of innate immune response regulation of immune response activation of immune response regulation of defense response regulation of response to biotic stimulus regulation of response to external stimulus innate immune response activating cell surface receptor signaling pathway positive regulation of response to stimulus positive regulation of response to external stimulus immune response-regulating signaling pathway regulation of response to stress innate immune response cell surface pattern recognition receptor signaling pathway regulation of signaling positive regulation of innate immune response defense response defense response to symbiont toll-like receptor 4 signaling pathway regulation of cell communication heart process negative regulation of heart rate system process regulation of heart contraction regulation of system process negative regulation of blood circulation negative regulation of heart contraction circulatory system process regulation of blood circulation heart contraction negative regulation of multicellular organismal process blood circulation regulation of heart rate regulation of tumor necrosis factor production positive regulation of tumor necrosis factor superfamily cytokine production cytokine production regulation of cytokine production tumor necrosis factor superfamily cytokine production positive regulation of tumor necrosis factor production tumor necrosis factor production regulation of tumor necrosis factor superfamily cytokine production positive regulation of cytokine production cellular response to interleukin-18 response to interleukin-18 response to cytokine cytokine-mediated signaling pathway interleukin-18-mediated signaling pathway cellular response to cytokine stimulus regulation of lamellipodium assembly regulation of lamellipodium organization lamellipodium assembly positive regulation of lamellipodium assembly positive regulation of lamellipodium organization lamellipodium organization response to estradiol regulation of blood pressure negative regulation of blood pressure cellular homeostasis chemical homeostasis homeostatic process glucose homeostasis carbohydrate homeostasis intracellular chemical homeostasis intracellular glucose homeostasis response to progesterone establishment of protein localization nitrogen compound transport nucleocytoplasmic transport transport nuclear transport organic substance transport intracellular transport protein localization to nucleus establishment of localization import into nucleus establishment of protein localization to organelle protein transport protein localization to organelle establishment of localization in cell protein import into nucleus intracellular protein transport regulation of leukocyte differentiation regulation of cell development regulation of osteoclast differentiation negative regulation of cell differentiation negative regulation of hemopoiesis negative regulation of leukocyte differentiation regulation of myeloid cell differentiation negative regulation of developmental process regulation of myeloid leukocyte differentiation negative regulation of myeloid cell differentiation negative regulation of osteoclast differentiation negative regulation of myeloid leukocyte differentiation negative regulation of cell development negative regulation of immune system process regulation of hemopoiesis positive regulation of establishment of protein localization regulation of intracellular transport positive regulation of protein localization to nucleus positive regulation of protein import into nucleus regulation of nucleocytoplasmic transport positive regulation of protein localization regulation of intracellular protein transport positive regulation of intracellular protein transport positive regulation of nucleocytoplasmic transport regulation of protein localization to nucleus positive regulation of protein transport regulation of protein import into nucleus positive regulation of intracellular transport regulation of transport regulation of establishment of protein localization regulation of protein transport positive regulation of transport extrinsic apoptotic signaling pathway via death domain receptors extrinsic apoptotic signaling pathway apoptotic signaling pathway monosaccharide metabolic process carbohydrate metabolic process hexose metabolic process small molecule metabolic process glucose metabolic process cellular response to growth hormone stimulus growth hormone receptor signaling pathway response to growth hormone cellular component disassembly regulation of focal adhesion disassembly focal adhesion disassembly cell junction disassembly cell-substrate junction disassembly positive regulation of cell-substrate junction organization positive regulation of focal adhesion disassembly cell-substrate junction organization regulation of cell-substrate junction organization DNA-templated transcription transcription by RNA polymerase II regulation of transcription by RNA polymerase II positive regulation of transcription by RNA polymerase II organic cyclic compound biosynthetic process regulation of DNA-templated transcription positive regulation of DNA-templated transcription RNA biosynthetic process nucleic acid biosynthetic process regulation of RNA biosynthetic process nucleobase-containing compound biosynthetic process positive regulation of RNA biosynthetic process response to acid chemical response to amino acid B cell activation cell activation mononuclear cell differentiation lymphocyte differentiation lymphocyte activation leukocyte activation B cell differentiation intracellular signal transduction intracellular signaling cassette phosphatidylinositol 3-kinase/protein kinase B signal transduction glycerophospholipid metabolic process lipid biosynthetic process phospholipid metabolic process phosphate-containing compound metabolic process phosphorus metabolic process phosphatidylinositol metabolic process glycerophospholipid biosynthetic process phosphatidylinositol phosphate biosynthetic process phospholipid biosynthetic process phosphatidylinositol biosynthetic process glycerolipid biosynthetic process organophosphate metabolic process organophosphate biosynthetic process cellular lipid metabolic process glycerolipid metabolic process lipid metabolic process negative regulation of cell population proliferation negative regulation of smooth muscle cell proliferation response to alcohol response to ethanol DNA damage response intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway response to light stimulus cellular response to radiation response to UV cellular response to environmental stimulus cellular response to light stimulus response to radiation response to abiotic stimulus cellular response to abiotic stimulus cellular response to UV muscle cell apoptotic process regulation of muscle cell apoptotic process negative regulation of muscle cell apoptotic process positive regulation of phosphorylation regulation of protein modification process regulation of protein phosphorylation positive regulation of protein metabolic process positive regulation of phosphate metabolic process positive regulation of protein modification process regulation of phosphate metabolic process positive regulation of protein phosphorylation protein modification process protein phosphorylation positive regulation of phosphorus metabolic process phosphorylation macromolecule modification regulation of phosphorus metabolic process regulation of phosphorylation cellular response to fatty acid cellular response to lipid response to nutrient levels response to nutrient positive regulation of cell communication positive regulation of signal transduction regulation of cellular response to stress endoplasmic reticulum unfolded protein response regulation of response to endoplasmic reticulum stress regulation of endoplasmic reticulum unfolded protein response positive regulation of response to endoplasmic reticulum stress positive regulation of endoplasmic reticulum unfolded protein response cellular response to topologically incorrect protein cellular response to unfolded protein response to unfolded protein response to topologically incorrect protein positive regulation of signaling	cell-cell junction anchoring junction cell junction cellular anatomical entity intracellular anatomical structure cytoplasm cytosol protein-containing complex catalytic complex membrane phosphatidylinositol 3-kinase complex, class I membrane protein complex transferase complex phosphatidylinositol 3-kinase complex, class IA cell-cell junction anchoring junction cell junction cellular anatomical entity intracellular anatomical structure cytoplasm cytosol protein-containing complex catalytic complex membrane phosphatidylinositol 3-kinase complex, class I membrane protein complex transferase complex phosphatidylinositol 3-kinase complex, class IA phosphatidylinositol 3-kinase complex transferase complex, transferring phosphorus-containing groups extrinsic component of membrane perinuclear endoplasmic reticulum membrane membrane-bounded organelle organelle subcompartment intracellular membrane-bounded organelle endomembrane system endoplasmic reticulum subcompartment intracellular organelle perinuclear endoplasmic reticulum perinuclear region of cytoplasm organelle nuclear outer membrane-endoplasmic reticulum membrane network endoplasmic reticulum organelle membrane endoplasmic reticulum membrane Golgi apparatus cis-Golgi network nucleus
B	DOUBLY PHOSPHORYLATED MIDDLE T ANTIGEN	-	-	host cellular component host cell part host cell membrane cellular anatomical entity membrane

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR035591	Phosphatidylinositol 3-kinase regulatory subunit alpha, SH3 domain	Domain
A	IPR032498	PI3K regulatory subunit p85-related , inter-SH2 domain	Domain
A	IPR036860	SH2 domain superfamily	Homologous Superfamily
A	IPR044124	PIK3R1, inter-SH2 domain	Domain
A	IPR036028	SH3-like domain superfamily	Homologous Superfamily
A	IPR008936	Rho GTPase activation protein	Homologous Superfamily
A	IPR035020	PI3K p85 subunit, C-terminal SH2 domain	Domain
A	IPR001452	SH3 domain	Domain
A	IPR035022	PI3K p85 subunit, N-terminal SH2 domain	Domain
A	IPR000980	SH2 domain	Domain
A	IPR000198	Rho GTPase-activating protein domain	Domain
B	IPR036092	Small/middle T-antigen superfamily	Homologous Superfamily
B	IPR001623	DnaJ domain	Domain
B	IPR003354	Small/middle T-antigen	Domain
B	IPR036869	Chaperone J-domain superfamily	Homologous Superfamily

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
B	PTR	Parent Component: TYR RESID: AA0039 PSI-MOD : O4'-phospho-L-tyrosine MOD:00048

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.